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Literature summary for 1.8.3.2 extracted from

  • Thorpe, C.; Hoober, K.L.; Raje, S.; Glynn, N.M.; Burnside, J.; Turi, G.K.; Coppock, D.L.
    Sulfhydryl oxidases: emerging catalysts of protein disulfide bond formation in eukaryotes (2002), Arch. Biochem. Biophys., 405, 1-12.
    View publication on PubMed
Search for more literature for 1.8.3.2

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme expression in endometrial glandular epithelium is inducible by estradiol in presence of cycloheximide, and by serum deprivation Cavia porcellus
additional information enzyme QSOX1 expression in fibroblasts is inducible by serum deprivation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, gene structure Gallus gallus
DNA and amino acid sequence determination and analysis, gene structure Cavia porcellus
DNA and amino acid sequence determination and analysis, gene structure Trypanosoma brucei
DNA and amino acid sequence determination and analysis, gene structure Rattus norvegicus
genes ERV1 and ERV2, DNA and amino acid sequence determination and analysis, gene structure, phylogenetic analysis Saccharomyces cerevisiae
genes QSCN6 or QSOX1, and QSOX2, DNA and amino acid sequence determination and analysis, QSCN6 or QSOX1 is located on chromosome 1q25.2, QSOX2 on chromosome 9q34, gene structure Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
Erv2p, X-ray diffraction structure determination and analysis Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the activity with small thiols is dominated by the Km value Gallus gallus
additional information
-
 additional information the activity with small thiols is dominated by the Km value Rattus norvegicus
0.0046
-
 O2
-
 Gallus gallus
0.02
-
 glutathione
-
 Gallus gallus
0.7
-
 dithiothreitol
-
 Rattus norvegicus
4.4
-
 glutathione
-
 Rattus norvegicus
6.9
-
 DTT Evr2p Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
apocrine cell
-
 Homo sapiens
-
-
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
endoplasmic reticulum
-
 Saccharomyces cerevisiae 5783
-
endoplasmic reticulum
-
 Homo sapiens 5783
-
extracellular
-
 Saccharomyces cerevisiae
-
-
extracellular egg white Gallus gallus
-
-
extracellular secretion by fungal mycelium Aspergillus niger
-
-
extracellular seminal vesicles Homo sapiens
-
-
Golgi apparatus
-
 Homo sapiens 5794
-
mitochondrial intermembrane space high concentration of Evr1p Saccharomyces cerevisiae 5758
-
nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains a FeS center Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 2 * 53000 Aspergillus niger
65000
-
 1 * 65000, about Rattus norvegicus
90000
-
 2 * 90000, about Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
insulin A and B chains + O2 Gallus gallus
-
 disulfide of insulin A and B chains + H2O2
-
 ?
lysozyme + O2 Gallus gallus
-
 lysozyme disulfide + H2O2
-
 ?
additional information Saccharomyces cerevisiae Evr1p is involved in cellular iron homeostasis, physiological role of the ERV1/ALR family enzymes, overview ?
-
 ?
additional information Gallus gallus preferred substrates are protein or peptide sulfhydryl groups, even of denatured cytoplasmic proteins, low molecular weight thiols, such as cysteine or glutathione, are poorer substrates ?
-
 ?
ovalbumin + O2 Gallus gallus
-
 ovalbumin disulfide + H2O2
-
 ?
R-SH + O2 Cavia porcellus
-
 R-S-S-R + H2O2
-
 ?
R-SH + O2 Saccharomyces cerevisiae
-
 R-S-S-R + H2O2
-
 ?
R-SH + O2 Aspergillus niger
-
 R-S-S-R + H2O2
-
 ir
R-SH + O2 Trypanosoma brucei
-
 R-S-S-R + H2O2
-
 ?
R-SH + O2 Gallus gallus enzyme plays a significant role in oxidative folding of a large variety of proteins R-S-S-R + H2O2
-
 ?
R-SH + O2 Rattus norvegicus enzyme plays a significant role in oxidative folding of a large variety of proteins R-S-S-R + H2O2
-
 ?
R-SH + O2 Homo sapiens enzyme plays a significant role in oxidative folding of a large variety of proteins R-S-S-R + H2O2
-
 ?
riboflavin-binding protein + O2 Gallus gallus
-
 riboflavin-binding protein disulfide + H2O2
-
 ?
RNase A + O2 Gallus gallus
-
 RNase A disulfide + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Cavia porcellus
-
-
-
Gallus gallus
-
-
-
Homo sapiens O00391 gene QSOX1
-
Rattus norvegicus Q6IUU3 gene QSOX1
-
Saccharomyces cerevisiae
-
 2 isozymes Evr1p and Evr2p
-
Trypanosoma brucei
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein highly glycosylated Gallus gallus

Reaction

Reaction Comment Organism Reaction ID
2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2 mechanistic scheme Gallus gallus
a
2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2 the CXXC motif in the active site sequence of Erv2p is catalytically essential, reaction mechanism involving reactive cysteine residues C121 and C124 of the A subunit, and C176 and C178 of the B subunit Saccharomyces cerevisiae
a

Source Tissue

Source Tissue Comment Organism Textmining
egg white
-
 Gallus gallus
-
endometrium glandular epithelial cells Cavia porcellus
-
epididymis high content Rattus norvegicus
-
epithelium endometrial glandular Cavia porcellus
-
fibroblast high expression level of QSOX1 Homo sapiens
-
additional information wide tissue distribution Gallus gallus
-
additional information tissue expression and distribution Rattus norvegicus
-
additional information tissue expression and distribution, high enzyme concentration in cell types associated with heavy secretory loads Homo sapiens
-
mycelium secretion of enzyme Aspergillus niger
-
oviduct secretory tissue Gallus gallus
-
seminal vesicle high content Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dithiothreitol + O2
-
 Gallus gallus dithiothreitol disulfide + H2O2
-
 ?
dithiothreitol + O2
-
 Rattus norvegicus dithiothreitol disulfide + H2O2
-
 r
dithiothreitol + O2 Evr2p, not Evr1p Saccharomyces cerevisiae dithiothreitol disulfide + H2O2
-
 ?
glutathione + O2
-
 Gallus gallus glutathione disulfide + H2O2
-
 ?
glutathione + O2
-
 Rattus norvegicus glutathione disulfide + H2O2
-
 ?
glutathione + O2 best small thiol substrate Aspergillus niger glutathione disulfide + H2O2
-
 ?
glutathione + O2 Evr2p, not Evr1p Saccharomyces cerevisiae glutathione disulfide + H2O2
-
 ?
insulin A and B chains + O2
-
 Gallus gallus disulfide of insulin A and B chains + H2O2
-
 ?
lysozyme + O2
-
 Gallus gallus lysozyme disulfide + H2O2
-
 ?
lysozyme + O2 Evr1p Saccharomyces cerevisiae lysozyme disulfide + H2O2
-
 ?
additional information Evr1p is involved in cellular iron homeostasis, physiological role of the ERV1/ALR family enzymes, overview Saccharomyces cerevisiae ?
-
 ?
additional information preferred substrates are protein or peptide sulfhydryl groups, even of denatured cytoplasmic proteins, low molecular weight thiols, such as cysteine or glutathione, are poorer substrates Gallus gallus ?
-
 ?
additional information low activity with reduced proteins Aspergillus niger ?
-
 ?
ovalbumin + O2
-
 Gallus gallus ovalbumin disulfide + H2O2
-
 ?
R-SH + O2
-
 Cavia porcellus R-S-S-R + H2O2
-
 ?
R-SH + O2
-
 Saccharomyces cerevisiae R-S-S-R + H2O2
-
 ?
R-SH + O2
-
 Aspergillus niger R-S-S-R + H2O2
-
 ir
R-SH + O2
-
 Trypanosoma brucei R-S-S-R + H2O2
-
 ?
R-SH + O2
-
 Rattus norvegicus R-S-S-R + H2O2
-
 ?
R-SH + O2
-
 Homo sapiens R-S-S-R + H2O2
-
 ?
R-SH + O2 enzyme plays a significant role in oxidative folding of a large variety of proteins Gallus gallus R-S-S-R + H2O2
-
 ?
R-SH + O2 enzyme plays a significant role in oxidative folding of a large variety of proteins Rattus norvegicus R-S-S-R + H2O2
-
 ?
R-SH + O2 enzyme plays a significant role in oxidative folding of a large variety of proteins Homo sapiens R-S-S-R + H2O2
-
 ?
R-SH + O2 best substrates are cysteine residues in reduced proteins Gallus gallus R-S-S-R + H2O2
-
 ?
riboflavin-binding protein + O2
-
 Gallus gallus riboflavin-binding protein disulfide + H2O2
-
 ?
RNase A + O2
-
 Gallus gallus RNase A disulfide + H2O2
-
 ?
RNase A + O2
-
 Rattus norvegicus RNase A disulfide + H2O2
-
 ?
RNase A + O2 low activity Aspergillus niger RNase A disulfide + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae
dimer 2 * 53000 Aspergillus niger
dimer 2 * 90000, about Gallus gallus
monomer 1 * 65000, about Rattus norvegicus
More structure Gallus gallus
More structure, active site structure containinbg a CXXC motif Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Erv1p
-
 Saccharomyces cerevisiae
ERv2p
-
 Saccharomyces cerevisiae
More enzyme belongs to the ERV1/ALR family Saccharomyces cerevisiae
More enzyme is a member of the Quiescin-sulfhydryl oxidase QSOX family Gallus gallus
More enzyme is a member of the Quiescin-sulfhydryl oxidase QSOX family Cavia porcellus
More enzyme is a member of the Quiescin-sulfhydryl oxidase QSOX family Trypanosoma brucei
More enzyme is a member of the Quiescin-sulfhydryl oxidase QSOX family Rattus norvegicus
More enzyme is a member of the Quiescin-sulfhydryl oxidase QSOX family Homo sapiens
QSCN6
-
 Homo sapiens
QSOX
-
 Gallus gallus
QSOX
-
 Cavia porcellus
QSOX
-
 Trypanosoma brucei
QSOX
-
 Rattus norvegicus
QSOx1
-
 Homo sapiens
QSOX2
-
 Homo sapiens
Quiescin Q6
-
 Homo sapiens
Quiescin-sulfhydryl oxidase
-
 Gallus gallus
Quiescin-sulfhydryl oxidase
-
 Cavia porcellus
Quiescin-sulfhydryl oxidase
-
 Trypanosoma brucei
Quiescin-sulfhydryl oxidase
-
 Rattus norvegicus
Quiescin-sulfhydryl oxidase
-
 Homo sapiens
sulfhydryl oxidase
-
 Gallus gallus
sulfhydryl oxidase
-
 Cavia porcellus
sulfhydryl oxidase
-
 Aspergillus niger
sulfhydryl oxidase
-
 Trypanosoma brucei
sulfhydryl oxidase
-
 Rattus norvegicus
sulfhydryl oxidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Gallus gallus
0.002
-
 RNAse A thiol per second Aspergillus niger
3.5
-
 DTT 3°C Gallus gallus
4
-
 DTT Evr2p Saccharomyces cerevisiae
20
-
 DTT 25°C Gallus gallus
66.67
-
 glutathione
-
 Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Aspergillus niger
FAD small FAD-binding domain Cavia porcellus
FAD small FAD-binding domain Trypanosoma brucei
FAD small FAD-binding domain Rattus norvegicus
FAD small FAD-binding domain Homo sapiens
FAD small FAD-binding domain, 1 molecule per subunit Gallus gallus
additional information enzyme contains a thioredoxin and a ERV1 domain Trypanosoma brucei
additional information enzyme contains a thioredoxin and an ERV1 domain Saccharomyces cerevisiae
additional information enzyme contains a thioredoxin domain Cavia porcellus
additional information enzyme contains a thioredoxin domain Rattus norvegicus
additional information enzyme contains a thioredoxin domain Homo sapiens
additional information enzyme contains a thioredoxin domain, 1 redox-active disulfide per subunit Gallus gallus
additional information redox-active disulfide involved in catalysis Aspergillus niger