KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
DTT | native enzyme, pH 7.5 | Gallus gallus | |
12.5 | - |
DTT | 60 kDa enzyme fragment, pH 7.5 | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, native enzyme, SDS-PAGE | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
R-SH + O2 | Gallus gallus | oxidation of protein or peptide sulfhydryl groups to disulfides with a concomitant reduction of molecular oxygen to peroxide | R-S-S-R + H2O2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | heavy glycosylation of the 60-kDa-enzyme fragment | Gallus gallus |
Purification (Comment) | Organism |
---|---|
intact enzyme and proteolytically cleaved enzyme in 30 kDa and 60 kDa fragments | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg white | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dithiothreitol + O2 | intact enzyme and 60-kDa-enzyme fragment | Gallus gallus | dithiothreitol disulfide + H2O2 | - |
ir | |
additional information | a 30 kDa enzyme fragment shows no catalytic activity of its own | Gallus gallus | ? | - |
? | |
R-SH + O2 | oxidation of protein or peptide sulfhydryl groups to disulfides with a concomitant reduction of molecular oxygen to peroxide | Gallus gallus | R-S-S-R + H2O2 | - |
ir | |
RNase A + O2 | intact enzyme, but not 60-kDa-enzyme fragment | Gallus gallus | RNase A disulfide + H2O2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, native enzyme, SDS-PAGE | Gallus gallus |
More | 2 enzyme fragments by partial proteolysis: a 30 kDa nonglycosylated monomeric fragment containing a thioredoxin domain with a CXXC motif, and a 60 kDa glycosylated dimeric fragment with bound FAD and catalytic activity, the latter different from intact enzyme activity | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family | Gallus gallus |
QSOX | - |
Gallus gallus |
sulfhydryl oxidase | - |
Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1013 | - |
DTT | native enzyme, pH 7.5 | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required for activity, binding domain is located on the 60-kDa-enzyme fragment, electron-transfer mechanism in the native enzyme and the 60 kDA enzyme fragment | Gallus gallus |