Any feedback?
Literature summary for 1.8.3.2 extracted from
- Expression of the secreted FAD-dependent sulfhydryl oxidase (QSOX) in the guinea pig central nervous system (2004), Mol. Brain Res., 125, 13-21.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cpQSOx1, primary gene sequence, DNA and amino acid sequence analysis, stable expression of the wild-type enzyme in MCF-7 cells with intra- and extracellular localization of the recombinant enzyme, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Cavia porcellus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | enzyme is secreted | Cavia porcellus | - |
- |
| perikaryon | - |
Cavia porcellus | 43204 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
x * 68000, recombinant His-tagged enzyme, SDS-PAGE | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| R-SH + O2 | Cavia porcellus | enzyme plays a role in synaptic strengthening and in redox activities in the brain | R-S-S-R + H2O2 | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | O08841 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel chelate chromatography | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| basal forebrain | - |
Cavia porcellus | - |
| brain | detailed tissue distribution analysis in brain, overview | Cavia porcellus | - |
| brain stem | - |
Cavia porcellus | - |
| central nervous system | highest enzyme expression in reticular structures, such as the basal forebrain, reticular thalamic nucleus, and reticular nuclei of the brainstem | Cavia porcellus | - |
| endometrium | specific expression in glandular and luminal epithelial cells | Cavia porcellus | - |
| epithelium | secretory, endometrial | Cavia porcellus | - |
| lung | - |
Cavia porcellus | - |
| neuron | - |
Cavia porcellus | - |
| ovary | - |
Cavia porcellus | - |
| secretory cell | - |
Cavia porcellus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dithiothreitol + O2 | - |
Cavia porcellus | dithiothreitol disulfide + H2O2 | - |
ir | |
| R-SH + O2 | - |
Cavia porcellus | R-S-S-R + H2O2 | - |
ir | |
| R-SH + O2 | enzyme plays a role in synaptic strengthening and in redox activities in the brain | Cavia porcellus | R-S-S-R + H2O2 | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, recombinant His-tagged enzyme, SDS-PAGE | Cavia porcellus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family | Cavia porcellus |
| QSOx1 | - |
Cavia porcellus |
| sulfhydryl oxidase | - |
Cavia porcellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cavia porcellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on | Cavia porcellus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
