Literature summary for 1.8.3.2 extracted from

Gross, E.; Sevier, C.S.; Heldman, N.; Vitu, E.; Bentzur, M.; Kaiser, C.A.; Thorpe, C.; Fass, D.
Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p (2006), Proc. Natl. Acad. Sci. USA, 103, 299-304.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain B(DE3)pLysS	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0001	-	O2	with 0.3 mM reduced thioredoxin as the substrate of the reductive half-reaction and an initial oxygen concentration of around 0.3 mM such that the reduced thioredoxin would be depleted by 10% during the course of the reaction	Saccharomyces cerevisiae
0.0004	-	O2	in the presence of 12.5 mM dithiothreitol	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48660	-	method not mentioned	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione-Sepharose column	Saccharomyces cerevisiae

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 R-SH + FAD	-	Saccharomyces cerevisiae	R-S-S-R + FADH2	-	?
dithiothreitol + O2	-	Saccharomyces cerevisiae	dithiothreitol disulfide + H2O2	-	?
R-SH + O2	-	Saccharomyces cerevisiae	R-S-S-R + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
Ero1p	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)