Cloned (Comment) | Organism |
---|---|
truncated version of Erv2p, without the N-terminal 34 residues, cloned into a pET24(a+) plasmid carrying kanamycin resistance. Amplified in DH5alpha Escherichia coli strain. Expressed from the Escherichia coli strain BL21(DE3) or BL21(DE3 star) | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | upon coordination with Zn2+, full reduction of Erv2p requires 6 electrons. Strongly inhibits Erv2p when assayed using tris(2-carboxyethyl)phosphine as the reducing substrate of the oxidase. Restoration of 1 mM EDTA effects rapid recovery of 80% of the original activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.3 | - |
dithiothreitol | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, Tris buffer, pH 7.5, 1 mM EDTA, more than 1 year | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis-(2-mercaptoethyl)sulfone + O2 | - |
Saccharomyces cerevisiae | ? + H2O | - |
? | |
dithiothreitol + O2 | - |
Saccharomyces cerevisiae | ? + H2O | - |
? | |
additional information | Erv2p is a modest catalyst of disulfide bond formation. None of the monothiols (at 10 mM), including beta-mercaptoethanol, N-acetylcysteamine, reduced glutathione and CoASH, prove detectable substrates of the yeast oxidase at pH 7.5. In contrast, dithiols are significant substrates | Saccharomyces cerevisiae | ? | - |
? | |
reduced lysozyme + O2 | - |
Saccharomyces cerevisiae | ? + H2O | - |
? | |
RNasered + O2 | - |
Saccharomyces cerevisiae | ? + H2O | - |
? | |
thioredoxin + O2 | - |
Saccharomyces cerevisiae | thioredoxin disulfide + H2O2 | - |
? | |
tris(2-carboxyethyl)-phosphine + O2 | - |
Saccharomyces cerevisiae | ? + H2O | - |
? | |
Trx Escherichia coli + O2 | - |
Saccharomyces cerevisiae | ? + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ERv2p | - |
Saccharomyces cerevisiae |
sulfhydryl oxidase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.43 | - |
dithiothreitol | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dithionite and photochemical reductions of Erv2p show full reduction of the flavin cofactor after the addition of 4 electrons with a midpoint potential of -200 mV at pH 7.5. No charge-transfer complex between a proximal thiolate and the oxidized flavin | Saccharomyces cerevisiae |