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Literature summary for 1.8.3.7 extracted from
- Copper is a cofactor of the formylglycine-generating enzyme (2017), ChemBioChem, 18, 161-165 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C187A/C231A/C284S/C298A | the mutant shows increased activity compared to the wild type enzyme | Thermomonospora curvata |
| C274S | inactive | Thermomonospora curvata |
| C296S | inactive | Thermomonospora curvata |
| S266A | the mutant shows reduced activity compared to the wild type enzyme | Thermomonospora curvata |
| S290K | the mutant shows very low activity compared to the wild type enzyme | Thermomonospora curvata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata |  |
| 0.52 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S266A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
| 0.58 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu+ | required | Thermomonospora curvata | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomonospora curvata | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + O2 + hydrogen sulfide + oxidized dithiothreitol | - |
Thermomonospora curvata | ? | - |
? | |
| additional information | low activity with Abz-SALSSPTRA-NH2 | Thermomonospora curvata | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FGE | - |
Thermomonospora curvata |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S266A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
| 0.027 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
| 0.07 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00011 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S290K, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
| 0.00082 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S266A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
| 0.048 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
| 0.333 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
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