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Literature summary for 1.8.3.7 extracted from

  • Holder, P.G.; Jones, L.C.; Drake, P.M.; Barfield, R.M.; Banas, S.; de Hart, G.W.; Baker, J.; Rabuka, D.
    Reconstitution of formylglycine-generating enzyme with copper(II) for aldehyde tag conversion (2015), J. Biol. Chem., 290, 15730-15745 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces coelicolor
expressed in Hi5 cells Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme contains a copper cofactor. The purified enzyme requires preactivation with copper Homo sapiens
Cu2+ the enzyme contains a copper cofactor. The purified enzyme requires preactivation with copper Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NBK3
-
-
Streptomyces coelicolor Q9F3C7
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap Excel resin column chromatography and Sephadex G-25 gel filtration Homo sapiens
Ni-NTA column chromatography and Sephadex G-25 gel filtration Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-AL-3-oxo-L-Ala-TPSRGSLFTGR-NH2 + hydrogen sulfide + oxidized dithiothreitol + H2O
-
 Homo sapiens ?
-
 ?
Ac-AL-3-oxo-L-Ala-TPSRGSLFTGR-NH2 + hydrogen sulfide + oxidized dithiothreitol + H2O
-
 Streptomyces coelicolor ?
-
 ?

Synonyms

Synonyms Comment Organism
FGE
-
 Homo sapiens
FGE
-
 Streptomyces coelicolor
sulfatase-modifying factor 1
-
 Homo sapiens
sulfatase-modifying factor 1
-
 Streptomyces coelicolor
SUMF1
-
 Homo sapiens
SUMF1
-
 Streptomyces coelicolor