BRENDA - Enzyme Database
show all sequences of 1.8.4.10

Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria

Bick, J.A.; Dennis, J.J.; Zylstra, G.J.; Nowack, J.; Leustek, T.; J. Bacteriol. 182, 135-142 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
not activated by sodium sulfate
Pseudomonas aeruginosa
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
5'-AMP
competitive vs. adenosine-5'-phosphosulfate
Pseudomonas aeruginosa
adenosine-5'-phosphosulfate
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00175
-
adenosine-5'-phosphosulfate
-
Pseudomonas aeruginosa
0.0196
-
thioredoxin
-
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-adenylyl sulfate + thioredoxin
Pseudomonas aeruginosa
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Pseudomonas putida
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Mycobacterium sp.
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Ralstonia pickettii
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Rhizobium tropici
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Burkholderia cepacia
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Pseudomonas aeruginosa
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Burkholderia cepacia DBO1 / ATCC 29424
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Burkholderia cepacia
Q9RFS6
-
-
Burkholderia cepacia DBO1 / ATCC 29424
Q9RFS6
-
-
Mycobacterium sp.
-
-
-
Pseudomonas aeruginosa
O05927
-
-
Pseudomonas putida
-
-
-
Ralstonia pickettii
-
-
-
Rhizobium tropici
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-adenylyl sulfate + thioredoxin
-
393947
Pseudomonas putida
AMP + sulfite + thioredoxin disulfide
-
393947
Pseudomonas putida
?
5'-adenylyl sulfate + thioredoxin
-
393947
Mycobacterium sp.
AMP + sulfite + thioredoxin disulfide
-
393947
Mycobacterium sp.
?
5'-adenylyl sulfate + thioredoxin
-
393947
Ralstonia pickettii
AMP + sulfite + thioredoxin disulfide
-
393947
Ralstonia pickettii
?
5'-adenylyl sulfate + thioredoxin
-
393947
Rhizobium tropici
AMP + sulfite + thioredoxin disulfide
-
393947
Rhizobium tropici
?
5'-adenylyl sulfate + thioredoxin
-
393947
Burkholderia cepacia
AMP + sulfite + thioredoxin disulfide
-
393947
Burkholderia cepacia
?
5'-adenylyl sulfate + thioredoxin
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
393947
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
393947
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
393947
Pseudomonas aeruginosa
?
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Pseudomonas putida
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Mycobacterium sp.
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Ralstonia pickettii
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Rhizobium tropici
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Burkholderia cepacia
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
-
393947
Burkholderia cepacia DBO1 / ATCC 29424
AMP + sulfite + thioredoxin disulfide
-
393947
Burkholderia cepacia DBO1 / ATCC 29424
?
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Burkholderia cepacia DBO1 / ATCC 29424
AMP + sulfite + thioredoxin disulfide
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
thioredoxin
-
Burkholderia cepacia
thioredoxin
-
Mycobacterium sp.
thioredoxin
5fold lower activity with dithiothreithol, 70fold lower activity with E. coli Grx1 or dithionite, 580fold lower activity with lipoic acid, no activity with ferredoxin
Pseudomonas aeruginosa
thioredoxin
-
Pseudomonas putida
thioredoxin
-
Ralstonia pickettii
thioredoxin
-
Rhizobium tropici
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
-
adenosine-5'-phosphosulfate
-
Pseudomonas aeruginosa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
not activated by sodium sulfate
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thioredoxin
-
Burkholderia cepacia
thioredoxin
-
Mycobacterium sp.
thioredoxin
5fold lower activity with dithiothreithol, 70fold lower activity with E. coli Grx1 or dithionite, 580fold lower activity with lipoic acid, no activity with ferredoxin
Pseudomonas aeruginosa
thioredoxin
-
Pseudomonas putida
thioredoxin
-
Ralstonia pickettii
thioredoxin
-
Rhizobium tropici
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5'-AMP
competitive vs. adenosine-5'-phosphosulfate
Pseudomonas aeruginosa
adenosine-5'-phosphosulfate
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
-
adenosine-5'-phosphosulfate
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00175
-
adenosine-5'-phosphosulfate
-
Pseudomonas aeruginosa
0.0196
-
thioredoxin
-
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-adenylyl sulfate + thioredoxin
Pseudomonas aeruginosa
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Pseudomonas putida
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Mycobacterium sp.
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Ralstonia pickettii
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Rhizobium tropici
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Burkholderia cepacia
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Pseudomonas aeruginosa
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
5'-adenylyl sulfate + thioredoxin
Burkholderia cepacia DBO1 / ATCC 29424
involved in assimilatory sulfate reduction
AMP + sulfite + thioredoxin disulfide
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-adenylyl sulfate + thioredoxin
-
393947
Pseudomonas putida
AMP + sulfite + thioredoxin disulfide
-
393947
Pseudomonas putida
?
5'-adenylyl sulfate + thioredoxin
-
393947
Mycobacterium sp.
AMP + sulfite + thioredoxin disulfide
-
393947
Mycobacterium sp.
?
5'-adenylyl sulfate + thioredoxin
-
393947
Ralstonia pickettii
AMP + sulfite + thioredoxin disulfide
-
393947
Ralstonia pickettii
?
5'-adenylyl sulfate + thioredoxin
-
393947
Rhizobium tropici
AMP + sulfite + thioredoxin disulfide
-
393947
Rhizobium tropici
?
5'-adenylyl sulfate + thioredoxin
-
393947
Burkholderia cepacia
AMP + sulfite + thioredoxin disulfide
-
393947
Burkholderia cepacia
?
5'-adenylyl sulfate + thioredoxin
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
393947
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
393947
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
393947
Pseudomonas aeruginosa
?
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Pseudomonas putida
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Mycobacterium sp.
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Ralstonia pickettii
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Rhizobium tropici
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Burkholderia cepacia
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
-
-
-
5'-adenylyl sulfate + thioredoxin
-
393947
Burkholderia cepacia DBO1 / ATCC 29424
AMP + sulfite + thioredoxin disulfide
-
393947
Burkholderia cepacia DBO1 / ATCC 29424
?
5'-adenylyl sulfate + thioredoxin
involved in assimilatory sulfate reduction
393947
Burkholderia cepacia DBO1 / ATCC 29424
AMP + sulfite + thioredoxin disulfide
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pseudomonas aeruginosa
Other publictions for EC 1.8.4.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743374
Paritala
Design, synthesis and evaluat ...
Mycobacterium tuberculosis
Nucleosides Nucleotides Nucleic Acids
34
199-220
2015
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741591
Paritala
A continuous spectrophotometr ...
Mycobacterium tuberculosis
Anal. Biochem.
440
32-39
2013
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1
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3
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3
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3
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2
2
742508
Stevenson
The X-ray crystal structure o ...
Physcomitrella patens
FEBS Lett.
587
3626-3632
2013
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1
1
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4
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1
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1
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4
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4
4
698950
Chung
Interaction domain on thioredo ...
Pseudomonas aeruginosa
J. Biol. Chem.
284
31181-31189
2009
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1
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1
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4
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699470
Hong
Identification of critical lig ...
Mycobacterium tuberculosis
J. Med. Chem.
52
5485-5495
2009
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667746
Kim
The two-domain structure of 5- ...
Pseudomonas aeruginosa
Biochemistry
46
591-601
2007
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669057
Gao
Noncovalent Complexes of APS R ...
Mycobacterium tuberculosis
J. Am. Soc. Mass Spectrom.
18
167-178
2007
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687585
Kopriva
The putative moss 3'-phosphoad ...
Physcomitrella patens
J. Biol. Chem.
282
22930-22938
2007
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5
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1
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694695
Wiedemann
The role of the novel adenosin ...
Physcomitrella patens
Plant Mol. Biol.
65
667-676
2007
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667787
Kim
The interaction of 5-adenylyls ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
1710
103-112
2005
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667852
Lopez-Cortes
-
Purification and preliminary c ...
Desulfovibrio aminophilus
Bioinorg. Chem. Appl.
3
81-91
2005
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1
2
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670496
Martin
The role of 5-adenylylsulfate ...
Pseudomonas aeruginosa
Photosyn. Res.
86
309-323
2005
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5
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636506
Williams
5'-Adenosinephosphosulfate lie ...
Bacillus subtilis, Mycobacterium tuberculosis, Mycolicibacterium smegmatis
J. Biol. Chem.
277
32606-32615
2002
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2
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2
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3
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2
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2
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4
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393947
Bick
Identification of a new class ...
Burkholderia cepacia, Burkholderia cepacia DBO1 / ATCC 29424, Mycobacterium sp., Pseudomonas aeruginosa, Pseudomonas putida, Ralstonia pickettii, Rhizobium tropici
J. Bacteriol.
182
135-142
2000
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1
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2
2
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8
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7
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15
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1
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6
1
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1
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1
6
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1
2
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8
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15
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1
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636505
Neumann
Characterization of the cys ge ...
Allochromatium vinosum
Mol. Biol. Rep.
27
27-33
2000
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1
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1
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1
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1
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288642
Prior
Structural and kinetic propert ...
Catharanthus roseus, Pisum sativum
Biochim. Biophys. Acta
1430
25-38
1999
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1
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4
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1
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2
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1
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4
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1
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1
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1
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2
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636502
Abola
Reduction of adenosine-5'-phos ...
Agrobacterium tumefaciens, Rhizobium leguminosarum, Rhizobium sp., Rhizobium sp. NGR234, Sinorhizobium fredii, Sinorhizobium meliloti
J. Bacteriol.
181
5280-5287
1999
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1
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1
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12
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7
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1
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18
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5
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5
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12
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18
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