BRENDA - Enzyme Database
show all sequences of 1.8.4.10

The X-ray crystal structure of APR-B, an atypical adenosine 5-phosphosulfate reductase from Physcomitrella patens

Stevenson, C.E.; Hughes, R.K.; McManus, M.T.; Lawson, D.M.; Kopriva, S.; FEBS Lett. 587, 3626-3632 (2013)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
crystal structure is consistent with a preference for adenosine 5'-phosphosulfate. The [Fe4-S4] cluster enhances the enzyme's activity
Physcomitrella patens
Engineering
Amino acid exchange
Commentary
Organism
D122G
mutant does not display improved activity with 3'-phosphoadenosine-5'-phosphosulfate and shows significantly reduced activity with 5'-adenylylsulfate as the substrate
Physcomitrella patens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
5'-adenylyl sulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.0069
-
5'-adenylyl sulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.04
-
3'-phosphoadenosine-5'-phosphosulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.049
-
3'-phosphoadenosine-5'-phosphosulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
gel filtration
Physcomitrella patens
71000
-
dynamic light scattering
Physcomitrella patens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Physcomitrella patens
Q8L5D0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenosine-5'-phosphosulfate + thioredoxin
substrate of phosphoadenylyl-sulfate reductase, EC 1.8.4.8
742508
Physcomitrella patens
? + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
-
742508
Physcomitrella patens
AMP + sulfite + thioredoxin disulfide
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
crystallization data
Physcomitrella patens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0001
-
3'-phosphoadenosine-5'-phosphosulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.0002
-
3'-phosphoadenosine-5'-phosphosulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.015
-
5'-adenylyl sulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.333
-
5'-adenylyl sulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
Cofactor
Cofactor
Commentary
Organism
Structure
[4Fe-4S]-center
-
Physcomitrella patens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[4Fe-4S]-center
-
Physcomitrella patens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure is consistent with a preference for adenosine 5'-phosphosulfate. The [Fe4-S4] cluster enhances the enzyme's activity
Physcomitrella patens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D122G
mutant does not display improved activity with 3'-phosphoadenosine-5'-phosphosulfate and shows significantly reduced activity with 5'-adenylylsulfate as the substrate
Physcomitrella patens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
5'-adenylyl sulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.0069
-
5'-adenylyl sulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.04
-
3'-phosphoadenosine-5'-phosphosulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.049
-
3'-phosphoadenosine-5'-phosphosulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
gel filtration
Physcomitrella patens
71000
-
dynamic light scattering
Physcomitrella patens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenosine-5'-phosphosulfate + thioredoxin
substrate of phosphoadenylyl-sulfate reductase, EC 1.8.4.8
742508
Physcomitrella patens
? + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
-
742508
Physcomitrella patens
AMP + sulfite + thioredoxin disulfide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
crystallization data
Physcomitrella patens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0001
-
3'-phosphoadenosine-5'-phosphosulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.0002
-
3'-phosphoadenosine-5'-phosphosulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.015
-
5'-adenylyl sulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.333
-
5'-adenylyl sulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0034
-
3'-phosphoadenosine-5'-phosphosulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.0037
-
3'-phosphoadenosine-5'-phosphosulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
2.6
-
5'-adenylyl sulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
48.3
-
5'-adenylyl sulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0034
-
3'-phosphoadenosine-5'-phosphosulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
0.0037
-
3'-phosphoadenosine-5'-phosphosulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
2.6
-
5'-adenylyl sulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
48.3
-
5'-adenylyl sulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
Physcomitrella patens
Other publictions for EC 1.8.4.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743374
Paritala
Design, synthesis and evaluat ...
Mycobacterium tuberculosis
Nucleosides Nucleotides Nucleic Acids
34
199-220
2015
-
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1
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2
-
-
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1
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1
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741591
Paritala
A continuous spectrophotometr ...
Mycobacterium tuberculosis
Anal. Biochem.
440
32-39
2013
-
1
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-
-
-
-
3
-
-
-
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1
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1
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3
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1
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1
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1
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3
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1
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3
-
-
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-
-
-
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-
2
2
742508
Stevenson
The X-ray crystal structure o ...
Physcomitrella patens
FEBS Lett.
587
3626-3632
2013
-
-
-
1
1
-
-
4
-
-
2
-
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3
-
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2
1
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4
-
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1
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1
1
1
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-
-
4
-
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2
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-
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-
2
1
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-
4
-
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-
-
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-
4
4
698950
Chung
Interaction domain on thioredo ...
Pseudomonas aeruginosa
J. Biol. Chem.
284
31181-31189
2009
-
-
-
-
-
1
-
1
-
-
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-
-
4
-
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1
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-
1
1
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1
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1
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1
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-
1
-
-
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-
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-
1
-
-
-
-
-
-
-
699470
Hong
Identification of critical lig ...
Mycobacterium tuberculosis
J. Med. Chem.
52
5485-5495
2009
-
-
-
-
-
-
1
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1
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1
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2
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2
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2
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1
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1
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2
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-
667746
Kim
The two-domain structure of 5- ...
Pseudomonas aeruginosa
Biochemistry
46
591-601
2007
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1
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2
-
1
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4
-
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1
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1
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2
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669057
Gao
Noncovalent Complexes of APS R ...
Mycobacterium tuberculosis
J. Am. Soc. Mass Spectrom.
18
167-178
2007
-
-
-
-
-
-
-
-
-
1
-
-
-
1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
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-
-
-
-
687585
Kopriva
The putative moss 3'-phosphoad ...
Physcomitrella patens
J. Biol. Chem.
282
22930-22938
2007
-
-
-
-
-
-
-
-
-
5
-
2
-
1
-
-
-
1
-
-
-
2
3
-
1
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
2
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
694695
Wiedemann
The role of the novel adenosin ...
Physcomitrella patens
Plant Mol. Biol.
65
667-676
2007
-
-
1
-
1
-
-
-
-
1
-
1
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
667787
Kim
The interaction of 5-adenylyls ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
1710
103-112
2005
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
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-
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5
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-
-
-
667852
Lopez-Cortes
-
Purification and preliminary c ...
Desulfovibrio aminophilus
Bioinorg. Chem. Appl.
3
81-91
2005
-
-
-
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
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-
-
1
-
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-
-
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-
1
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1
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-
1
2
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1
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-
1
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-
-
-
-
-
-
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-
-
-
-
-
670496
Martin
The role of 5-adenylylsulfate ...
Pseudomonas aeruginosa
Photosyn. Res.
86
309-323
2005
-
-
1
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-
-
-
-
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-
-
5
-
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1
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-
-
-
-
636506
Williams
5'-Adenosinephosphosulfate lie ...
Bacillus subtilis, Mycobacterium tuberculosis, Mycolicibacterium smegmatis
J. Biol. Chem.
277
32606-32615
2002
-
-
2
-
-
-
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-
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2
-
3
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4
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2
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2
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-
4
-
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-
-
393947
Bick
Identification of a new class ...
Burkholderia cepacia, Burkholderia cepacia DBO1 / ATCC 29424, Mycobacterium sp., Pseudomonas aeruginosa, Pseudomonas putida, Ralstonia pickettii, Rhizobium tropici
J. Bacteriol.
182
135-142
2000
1
-
1
-
-
-
2
2
-
-
-
8
-
7
-
-
-
-
-
-
-
-
15
-
-
-
-
-
1
-
-
6
1
-
-
1
-
1
6
-
-
-
-
2
1
2
-
-
-
8
-
-
-
-
-
-
-
-
15
-
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-
-
1
-
-
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-
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-
-
-
-
636505
Neumann
Characterization of the cys ge ...
Allochromatium vinosum
Mol. Biol. Rep.
27
27-33
2000
-
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1
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1
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1
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1
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1
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288642
Prior
Structural and kinetic propert ...
Catharanthus roseus, Pisum sativum
Biochim. Biophys. Acta
1430
25-38
1999
-
-
1
-
-
-
-
4
-
-
1
-
-
2
-
-
1
-
-
1
-
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2
1
-
-
-
2
-
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-
1
-
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-
-
-
-
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4
-
-
1
-
-
-
-
1
-
1
-
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
636502
Abola
Reduction of adenosine-5'-phos ...
Agrobacterium tumefaciens, Rhizobium leguminosarum, Rhizobium sp., Rhizobium sp. NGR234, Sinorhizobium fredii, Sinorhizobium meliloti
J. Bacteriol.
181
5280-5287
1999
-
-
1
-
-
-
2
1
-
-
-
12
-
7
-
-
1
-
-
-
-
-
18
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
5
-
-
-
-
2
-
1
-
-
-
12
-
-
-
1
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-