Cloned (Comment) | Organism |
---|---|
gene msrB with the codon for selenomehinonine is exchanged for methionine, overexpression in Escherichia coli | Mus musculus |
pilB is transcribed as polycistronic transcript, overexpression of PilB as His-tagged protein in Escherichia coliBL21(DE3) | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
MsrB is a selenoprotein | - |
Staphylococcus aureus | - |
PilB | - |
Purification (Comment) | Organism |
---|---|
partial purification of native MsrB from liver, recombinant modified MsrB from Escherichia coli | Mus musculus |
recombinant His-tagged PilB from Escherichia coli by nickel affinity chromatography | Staphylococcus aureus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
cerebellum | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | highest activity | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
tissue specific activity, activity in tissues of MsrA-deficient mutant mice | Mus musculus |
0.0007 | - |
kidney, substrate L-methionine (R)-sulfoxide | Mus musculus |
0.0019 | - |
liver, substrate L-methionine (R)-sulfoxide | Mus musculus |
1.78 | - |
purified recombinant PilB, substrate L-methionine (R)-sulfoxide | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (R)-sulfoxide + thioredoxin | the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form | Mus musculus | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | PilB shows absolute specificity for the R-form of free and protein-bound methionine sulfoxide | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | the native MsrB as well as the recombinant modified MsrB show absolute specificity for the R-form of free and protein-bound methionine sulfoxide, no activity with the S-form | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Staphylococcus aureus |
methionine sulfoxide reductase | - |
Mus musculus |
MsrB | - |
Staphylococcus aureus |
MsrB | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Staphylococcus aureus |
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Staphylococcus aureus | |
thioredoxin | - |
Mus musculus |