Cloned (Comment) | Organism |
---|---|
overexpression of truncated wild-type and mutant enzymes as His-tagged proteins in Escherichia coli, functional coexpression of CBS1 in oocytes with Drosophila melanogaster ShC/B potassium channel | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C105S | site-directed mutagenesis, unaltered activity compared to the wild-type enzyme | Homo sapiens |
C169S | site-directed mutagenesis, active site mutant, completely inactive mutant | Homo sapiens |
W110A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | selenoprotein | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein L-methionine (R)-sulfoxide + thioredoxin | Homo sapiens | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | protein L-methionine + thioredoxin disulfide | - |
? | |
protein-L-methionine (R)-sulfoxide + dithiothreitol | Homo sapiens | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | protein-L-methionine + dithiothreitol disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | the active site selenocysteine SeC169 is essential for enzyme activity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | most abundant in ventricles, interventricular septum, and apex | Homo sapiens | - |
heart ventricle | - |
Homo sapiens | - |
interventricular septum | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
additional information | tissue distribution, main expression of CBS1 in muscle tissues, overview | Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein L-methionine (R)-sulfoxide + thioredoxin | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | Homo sapiens | protein L-methionine + thioredoxin disulfide | - |
? | |
protein-L-methionine (R)-sulfoxide + dithiothreitol | type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins | Homo sapiens | protein-L-methionine + dithiothreitol disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBS1 | - |
Homo sapiens |
MsrB | - |
Homo sapiens |
peptide methionine sulfoxide reductase type B | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Homo sapiens | |
thioredoxin | - |
Homo sapiens |