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Literature summary for 1.8.4.12 extracted from

  • Aachmann, F.L.; Kwak, G.H.; Del Conte, R.; Kim, H.Y.; Gladyshev, V.N.; Dikiy, A.
    Structural and biochemical analysis of mammalian methionine sulfoxide reductase B2 (2011), Proteins, 79, 3123-3131.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.12

Protein Variants

Protein Variants Comment Organism
C98S the mutant show increased Km values with dithiothreitol (1.7fold) and thioredoxin (6fold) compared to the wild type enzyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 dithiothreitol wild type enzyme, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus
0.05
-
 dithiothreitol mutant enzyme C98S, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus
0.66
-
 thioredoxin wild type enzyme, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus
7
-
 thioredoxin mutant enzyme C98S, in 50 mM sodium phosphate, pH 7.5, 50 mM NaCl, at 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q78J03
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.023
-
 in 100 mM sodium phosphate, at 37°C and pH 5.7 Mus musculus
0.055
-
 in 100 mM sodium phosphate, at 37°C and pH 6.5 Mus musculus
0.074
-
 in 50 mM carbonate-bicarbonate, at 37°C and pH 10.0 Mus musculus
0.11
-
 in 100 mM sodium phosphate, at 37°C and pH 7.0 Mus musculus
0.119
-
 in 50 mM Tris-HCl, at 37°C and pH 7.6 Mus musculus
0.228
-
 in 100 mM sodium phosphate, at 37°C and pH 7.5 Mus musculus
0.267
-
 in 50 mM Tris-HCl, at 37°C and pH 8.0 Mus musculus
0.272
-
 in 50 mM carbonate-bicarbonate, at 37°C and pH 9.6 Mus musculus
0.446
-
 in 50 mM Tris-HCl, at 37°C and pH 8.6 Mus musculus
0.462
-
 in 50 mM carbonate-bicarbonate, at 37°C and pH 9.2 Mus musculus
0.484
-
 in 50 mM Tris-HCl, at 37°C and pH 9.0 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-sulfoxide + dithiothreitol
-
 Mus musculus dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
dabsyl-L-methionine (R)-sulfoxide + thioredoxin
-
 Mus musculus dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B2
-
 Mus musculus
MsrB2
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Mus musculus