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Literature summary for 1.8.4.13 extracted from

  • Ejiri, S.; Weissbach, H.; Brot, N.
    The purification of methionine sulfoxide reductase from Escherichia coli (1980), Anal. Biochem., 102, 393-398.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
L-methionine (S)-S-oxide pH 7.5, 37┬░C Escherichia coli B

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli B

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
x * 21000, SDS-PAGE Escherichia coli B

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli B methionine sulfoxide reduction pathway, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme about 1100fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, again anion exchange chromatography, heat treatment, and hydroxyapatite chromatography to near homogeneity Escherichia coli B

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.00452
-
purified enzyme Escherichia coli B

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (S)-S-oxide + NADPH
-
Escherichia coli B L-methionine + NADP+ + H2O
-
?
L-methionine (S)-S-oxide + reduced DTT highest activity at 10 mM Escherichia coli B L-methionine + oxidized DTT + H2O
-
?
additional information methionine sulfoxide reduction pathway, overview Escherichia coli B ?
-
?

Subunits

Subunits Comment Organism
? x * 21000, SDS-PAGE Escherichia coli B

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase
-
Escherichia coli
methionine sulfoxide reductase
-
Escherichia coli B

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Escherichia coli B

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli B

Cofactor

Cofactor Comment Organism Structure
NADPH required, can be substituted by DTT, but not by GSH or 2-mercaptoethanol Escherichia coli B