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Literature summary for 1.8.4.13 extracted from

  • Black, S.; Harte, E.M.; Hudson, B.; Wartolofsky, L.
    A specific enzymatic reduction of L(-)methionine sulfoxide and a related nonspecific reduction of disulfides (1960), J. Biol. Chem., 235, 2910-2916.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inhibition at 1 mM Saccharomyces cerevisiae
arsenite strong inhibition Saccharomyces cerevisiae
bovine serum albumin
-
Saccharomyces cerevisiae
DL-methionine sulfoximine 50% inhibition at 1 mM Saccharomyces cerevisiae
hydroxyethyl disulfide competitively inhibits the activity with L-(-)-methionine S-oxide about 2.5fold Saccharomyces cerevisiae
iodoacetic acid complete inhibition at 10 mM Saccharomyces cerevisiae
L-(-)-methionine S-oxide competitively inhibits the activity with a disulfide about 2.5fold Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
L-(-)-methionine S-oxide pH 7.0, 22┬░C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-(-)-methionine S-oxide + NADPH Saccharomyces cerevisiae the enzyme is specific for the L-(-)-stereoisomer L-methionine + NADP+ + H2O
-
ir
additional information Saccharomyces cerevisiae possible pathway, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
two enzyme forms
-

Purification (Commentary)

Purification (Comment) Organism
two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-(-)-methionine S-oxide + NADPH the enzyme is specific for the L-(-)-stereoisomer Saccharomyces cerevisiae L-methionine + NADP+ + H2O
-
ir
L-(-)-methionine S-oxide + NADPH the enzyme is absolutely specific for the L-(-)-stereoisomer Saccharomyces cerevisiae L-methionine + NADP+ + H2O
-
ir
additional information possible pathway, overview Saccharomyces cerevisiae ?
-
?
additional information the purified enzyme may contain thioredoxin Saccharomyces cerevisiae ?
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
22
-
assay at room temperature Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH specific for Saccharomyces cerevisiae