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Literature summary for 1.8.4.2 extracted from

  • Pace, M.; Dixon, J.E.
    The nature of the multiple forms of bovine thiol:protein disulfide oxidoreductase (1979), Int. J. Pept. Protein Res., 14, 409-413.
    View publication on PubMed

General Stability

General Stability Organism
dimerization after prolonged storage at -20°C, freeze-thawing or heating at 60°C Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
1 * 60000, SDS-PAGE Bos taurus
60000
-
2 * 60000, SDS-PAGE, dimerization after prolonged storage at -20°C, freeze-thawing or heating at 60°C, monomers held together by an intermolecular disulfide bond Bos taurus
120000
-
MW 180000 also found in traces, monomer has MW 60000 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSH + insulin
-
Bos taurus GSSG + reduced insulin chain A and B
-
?
GSH + protein disulfide
-
Bos taurus GSSG + protein-dithiol
-
r

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE, dimerization after prolonged storage at -20°C, freeze-thawing or heating at 60°C, monomers held together by an intermolecular disulfide bond Bos taurus
monomer 1 * 60000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus