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Literature summary for 1.8.4.2 extracted from
- Periplasmic protein thiol:disulfide oxidoreductases of Escherichia coli (2000), FEMS Microbiol. Rev., 24, 303-316.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | DsbA protein is a target for treatment of pathogenic bacteria | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | DsbA and DsbB insertion mutants are sensitive to dithiothreitol and benzylpenicillin or Cd2+, Hg2+ and Zn2+, pleitropic phenotype | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | outer cytoplasmic membrane, protein termini localised in the periplasm | Escherichia coli | 16020 | - |
| periplasm | DsbA protein soluble, DsbB protein is membrane intrinsic | Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
DsbB gene product | Escherichia coli |
| 21100 | - |
DsbA gene product | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSH + protein disulfide | Escherichia coli | disulfide bonding step in folding pathway of many periplasmic and outer membrane proteins with structural disulfide bonds | GSSG + protein-dithiol | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
DsbA homologous | - |
| Dickeya chrysanthemi DsbA homologous | - |
DsbA homologous | - |
| Escherichia coli | - |
in periplasm: DsbA and DsbB enzymes | - |
| Escherichia coli | - |
different forms of protein thiol:disulfide oxidoreductases with either oxidizing, gene DsbA, reducing, gene DsbB, or isomerising, gene DsbC and DsbD, activity exist in Escherichia coli | - |
| Haemophilus influenzae | - |
DsbA homologous | - |
| Haemophilus influenzae DsbA homologous | - |
DsbA homologous | - |
| Klebsiella sp. | - |
DsbA homologous | - |
| Klebsiella sp. DsbA homologous | - |
DsbA homologous | - |
| Legionella pneumophila | P50024 | DsbA homologous | - |
| Legionella pneumophila DsbA homologous | P50024 | DsbA homologous | - |
| Lelliottia amnigena | Q9XDP0 | DsbA homologous | - |
| Lelliottia amnigena DsbA homologous | Q9XDP0 | DsbA homologous | - |
| Pectobacterium carotovorum | - |
DsbA homologous | - |
| Pectobacterium carotovorum DsbA homologous | - |
DsbA homologous | - |
| Pseudomonas aeruginosa | P0C2B2 | DsbA homologous | - |
| Pseudomonas aeruginosa DsbA homologous | P0C2B2 | DsbA homologous | - |
| Pseudomonas syringae | O52376 | DsbA homologous | - |
| Pseudomonas syringae DsbA homologous | O52376 | DsbA homologous | - |
| Salmonella enterica subsp. enterica serovar Enteritidis | - |
DsbA homologous | - |
| Salmonella enterica subsp. enterica serovar Enteritidis DsbA homologous | - |
DsbA homologous | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
DsbA homologous | - |
| Salmonella enterica subsp. enterica serovar Typhimurium DsbA homologous | - |
DsbA homologous | - |
| Shigella flexneri | - |
DsbA homologous | - |
| Shigella flexneri DsbA homologous | - |
DsbA homologous | - |
| Vibrio cholerae serotype O1 | - |
DsbA homologous | - |
| Vibrio cholerae serotype O1 DsbA homologous | - |
DsbA homologous | - |
| Yersinia pestis | - |
DsbA homologous | - |
| Yersinia pestis DsbA homologous | - |
DsbA homologous | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol | C-X-X-C motif in active site for disulfide bonding, either oxidizing, reducing and isomerising activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSH + protein disulfide | very rapid disulfide interchange reaction | Escherichia coli | GSSG + protein-dithiol | - |
r | |
| GSH + protein disulfide | disulfide bonding step in folding pathway of many periplasmic and outer membrane proteins with structural disulfide bonds | Escherichia coli | GSSG + protein-dithiol | - |
r | |
| additional information | DsbB protein re-oxidizes the reduced DsbA protein | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21100 | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | DsbA protein family including periplasmic protein disulfide: thiol oxidoreductases with high homology in amino acid sequences | Escherichia coli |
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