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Literature summary for 1.8.4.2 extracted from

  • Zaffagnini, M.; Bedhomme, M.; Marchand, C.H.; Couturier, J.; Gao, X.-H.; Rouhier, N.; Trost, P.; Lemaire, S.D.
    Glutaredoxin s12 unique properties for redox signaling (2012), Antioxid. Redox Signal., 16, 17-32 .
    View publication on PubMed
Search for more literature for 1.8.4.2

Protein Variants

Protein Variants Comment Organism
C29S specific activity about 5% of wild-type Populus sp.
C87S specific activity simiular to wild-type Populus sp.

Inhibitors

Inhibitors Comment Organism Structure
H2O2
-
 Populus sp.
iodoacetamide
-
 Populus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 glutathionylated BSA mutant C87S, pH not specified in the publication, temperature not specified in the publication Populus sp.
0.03
-
 glutathionylated BSA wild-type, pH not specified in the publication, temperature not specified in the publication Populus sp.
0.3
-
 2-mercaptoethanol glutathione disulfide wild-type, pH not specified in the publication, temperature not specified in the publication Populus sp.
0.31
-
 2-mercaptoethanol glutathione disulfide mutant C87S, pH not specified in the publication, temperature not specified in the publication Populus sp.
3.1
-
 glutathione mutant C87S, pH not specified in the publication, temperature not specified in the publication Populus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Populus sp. 9507
-

Organism

Organism UniProt Comment Textmining
Populus sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66
-
 wild-type, pH not specified in the publication, temperature not specified in the publication Populus sp.
70
-
 wild-type, pH not specified in the publication, temperature not specified in the publication Populus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-mercaptoethanol glutathione disulfide + glutathione
-
 Populus sp. 2-mercaptoethanol disulfide + glutathione disulfide
-
 ?
glutathionylated BSA + glutathione
-
 Populus sp. BSA + glutathione disulfide
-
 ?
glutathionylated isocitrate lyase + glutathione substrate is glutathionylated isocitrate lyase of Chlamydomonas rheinhardtii Populus sp. glutathione disulfide + isocitrate lyase
-
 ?

Synonyms

Synonyms Comment Organism
GrxS12
-
 Populus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
 2-mercaptoethanol glutathione disulfide wild-type, pH not specified in the publication, temperature not specified in the publication Populus sp.
26.5
-
 2-mercaptoethanol glutathione disulfide mutant C87S, pH not specified in the publication, temperature not specified in the publication Populus sp.
31
-
 glutathionylated BSA wild-type, pH not specified in the publication, temperature not specified in the publication Populus sp.
33.9
-
 glutathionylated BSA mutant C87S, pH not specified in the publication, temperature not specified in the publication Populus sp.
92.8
-
 glutathione wild-type, pH not specified in the publication, temperature not specified in the publication Populus sp.
102.8
-
 glutathione mutant C87S, pH not specified in the publication, temperature not specified in the publication Populus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 maximum activity above pH 9 Populus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
 reaction is negligible Populus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.112
-
 H2O2 pH not specified in the publication, temperature not specified in the publication Populus sp.
0.114
-
 iodoacetamide pH not specified in the publication, temperature not specified in the publication Populus sp.

General Information

General Information Comment Organism
metabolism enzyme reacts with glutathionylated substrates in a GSH-dependent ping pong mechanism. The pKa of GrxS12 catalytic Cys29 is very low (3.9) and makes GrxS12 itself sensitive to oxidation by H2O2 and to direct glutathionylation by nitrosoglutathione. Glutathionylated-GrxS12 is temporarily inactive until it is deglutathionylated by GSH Populus sp.