BRENDA - Enzyme Database
show all sequences of 1.8.4.4

Thiol-disulfide transhydrogenase (yeast)

Nagai, S.; Methods Enzymol. 17B, 510-515 (1971)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
ethylene glycol, 10%, stabilizes
Saccharomyces cerevisiae
glutathione stabilizes, stabilizing effect increased by cystine
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell associated
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cystine + GSH
Saccharomyces cerevisiae
-
cysteine + GSSG
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
61.6
-
beta-hydroxyethyldisulfide
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
-20°C, pH 5.8, 1 mM glutathione, several months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cystine + GSH
-
395015
Saccharomyces cerevisiae
cysteine + GSSG
-
-
-
?
additional information
inactive with: insulin and other proteins
395015
Saccharomyces cerevisiae
?
-
-
-
?
additional information
at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances
395015
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
54
-
crude state, quite labile to heat above 54°C
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate
Saccharomyces cerevisiae
General Stability (protein specific)
General Stability
Organism
ethylene glycol, 10%, stabilizes
Saccharomyces cerevisiae
glutathione stabilizes, stabilizing effect increased by cystine
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell associated
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cystine + GSH
Saccharomyces cerevisiae
-
cysteine + GSSG
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
61.6
-
beta-hydroxyethyldisulfide
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, pH 5.8, 1 mM glutathione, several months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cystine + GSH
-
395015
Saccharomyces cerevisiae
cysteine + GSSG
-
-
-
?
additional information
inactive with: insulin and other proteins
395015
Saccharomyces cerevisiae
?
-
-
-
?
additional information
at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances
395015
Saccharomyces cerevisiae
?
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
54
-
crude state, quite labile to heat above 54°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate
Saccharomyces cerevisiae
Other publictions for EC 1.8.4.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
395016
Minoda
-
Thiol-disulfide transhydrogena ...
Saccharomyces cerevisiae
Agric. Biol. Chem.
37
2511-2516
1973
1
-
-
-
-
-
9
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
9
-
-
-
-
-
1
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395019
States
Interrelationship of glutathio ...
Rattus norvegicus
Biochem. J.
132
623-631
1973
-
-
-
-
-
-
-
2
1
-
-
1
-
3
-
-
-
-
-
2
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
-
-
-
2
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
395015
Nagai
-
Thiol-disulfide transhydrogena ...
Saccharomyces cerevisiae
Methods Enzymol.
17B
510-515
1971
-
-
-
-
-
2
-
-
1
-
-
1
-
1
-
-
1
-
-
-
1
1
3
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
1
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
395018
States
Distribution of glutathione-cy ...
Rattus norvegicus
Biochem. J.
113
443-444
1969
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395014
Nagai
A thiol-disulfide transhydroge ...
Saccharomyces cerevisiae
J. Biol. Chem.
243
1942-1947
1968
-
-
-
-
-
6
-
-
1
-
1
1
-
1
-
-
1
-
-
-
1
1
9
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
1
1
-
-
-
1
-
-
1
1
9
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
395017
Wendell
Distribution of glutathione re ...
Rattus norvegicus
Biochim. Biophys. Acta
159
179-181
1968
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-