Literature summary for 1.8.4.9 extracted from

Phartiyal, P.; Kim, W.S.; Cahoon, R.E.; Jez, J.M.; Krishnan, H.B.
The role of 5-adenylylsulfate reductase in the sulfur assimilation pathway of soybean: molecular cloning, kinetic characterization, and gene expression (2008), Phytochemistry, 69, 356-364.

Search for more literature for 1.8.4.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Glycine max

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	competitive inhibitor with respect to 5'-adenylyl sulfate	Glycine max
additional information	sulfite (up to 100 mM) has no inhibitory effect	Glycine max

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0112	-	5'-adenylyl sulfate	30°C, pH 8.5, cosubstrate: dithiothreitol	Glycine max
0.0174	-	5'-adenylyl sulfate	30°C, pH 8.5, cosubstrate: glutathione	Glycine max
0.495	-	glutathione	30°C, pH 8.5	Glycine max
0.595	-	dithiothreitol	30°C, pH 8.5	Glycine max

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	2 * 46000, SDS-PAGE	Glycine max

Organism

Organism	UniProt	Comment	Textmining
Glycine max	-	L. Merr., cv Williams 82	-

Purification (Commentary)

Purification (Comment)	Organism
-	Glycine max

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	cold-treatment increases expression and the total activity of APS reductase in root tissues	Glycine max	-
seed	sulfur and phosphorus deprivation increases expression level, while nitrogen starvation represses APS reductase mRNA transcript and protein levels	Glycine max	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.207	-	-	Glycine max

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-adenylyl sulfate + glutathione	-	Glycine max	AMP + sulfite + glutathione disulfide	-	?
5'-adenylylsulfate + dithiothreitol	-	Glycine max	adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 46000, SDS-PAGE	Glycine max

Synonyms

Synonyms	Comment	Organism
5'-adenylylsulfate reductase	-	Glycine max
APS reductase	-	Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.4	-	glutathione	30°C, pH 8.5	Glycine max
8.4	-	5'-adenylyl sulfate	30°C, pH 8.5, cosubstrate: dithiothreitol	Glycine max
10.1	-	dithiothreitol	30°C, pH 8.5	Glycine max
11.3	-	5'-adenylyl sulfate	30°C, pH 8.5, cosubstrate: glutathione	Glycine max

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1	-	AMP	-	Glycine max

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Release 2023.1 (January 2023)