Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Nicotiana tabacum | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15200 | - |
x * 15200, calculated from sequence | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-methionine-(R)-S-sulfoxide + thionein | Nicotiana tabacum | - |
peptide-L-methionine + thionein disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | A7U630 | precursor, fragment | - |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein | NtMsrB1 | Nicotiana tabacum | dabsyl-L-methionine + bovine liver thionein + H2O | - |
? | |
dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol | NtMsrB1 | Nicotiana tabacum | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32 | NtMsrB1 | Nicotiana tabacum | dabsyl-L-methionine + ? | - |
? | |
dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine | NtMsrB1 | Nicotiana tabacum | dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O | - |
? | |
additional information | NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction | Nicotiana tabacum | ? | - |
? | |
peptide-L-methionine-(R)-S-sulfoxide + thionein | - |
Nicotiana tabacum | peptide-L-methionine + thionein disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15200, calculated from sequence | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
NtMsrB1 | - |
Nicotiana tabacum |
thioredoxin-independent methionine sulfoxide reductases B | - |
Nicotiana tabacum |