Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli NiCo21(DE3) cells | Oryza sativa Japonica Group |
Crystallization (Comment) | Organism |
---|---|
native, ascorbate-bound, and glutathione-bound enzyme forms, hanging drop vapor diffusion method, using 0.15 M potassium bromide and 30% (w/v) PEG MME 2000 | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
K20A | inactive | Oryza sativa Japonica Group |
K47A | the mutant shows about wild type activity | Oryza sativa Japonica Group |
K8A | the mutation significantly reduces the enzymatic activity | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | Oryza sativa Japonica Group | - |
glutathione disulfide + ascorbate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q65XA0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Oryza sativa Japonica Group |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | - |
Oryza sativa Japonica Group | glutathione disulfide + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Oryza sativa Japonica Group |
Synonyms | Comment | Organism |
---|---|---|
dehydroascorbate reductase | - |
Oryza sativa Japonica Group |
DHAR | - |
Oryza sativa Japonica Group |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme retains more than 80% activity between 30 and 60°C for 20 min | Oryza sativa Japonica Group |