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Literature summary for 1.8.5.1 extracted from
- Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii kinetics and specificity in deglutathionylation reactions (2010), FEBS Lett., 584, 2242-2248 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii |  |
| 0.39 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.96 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 1.63 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Chlamydomonas reinhardtii | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
PAGE | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | A8IYH1 | isoform GRX2 | - |
| Chlamydomonas reinhardtii | A8JHA9 | isoform GRX1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 glutathione + dehydroascorbate | - |
Chlamydomonas reinhardtii | glutathione disulfide + ascorbate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 12000, SDS-PAGE | Chlamydomonas reinhardtii |
| More | formation of an intramolecular disulfide between Cys27 and Cys30 within the CPYC active site of GRX2 | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GRX1 | - |
Chlamydomonas reinhardtii |
| GRX2 | - |
Chlamydomonas reinhardtii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.84 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.94 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 1.67 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 1.82 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.98 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 1.11 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 4.3 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 4.92 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
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