Protein Variants | Comment | Organism |
---|---|---|
A178Q | mutation in subunit DmsA. About 1200% of wild-type catalytic efficiency | Escherichia coli |
A178Q | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
A181T | mutation in subunit DmsA. About 300% of wild-type catalytic efficiency | Escherichia coli |
G167N | mutation in subunit DmsA. About 20% of wild-type catalytic efficiency | Escherichia coli |
G167N | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
G190D | mutation in subunit DmsA. About 80% of wild-type catalytic efficiency | Escherichia coli |
G190V | mutation in subunit DmsA. About 180% of wild-type catalytic efficiency | Escherichia coli |
M147I | mutation in subunit DmsA. About 65% of wild-type catalytic efficiency | Escherichia coli |
M147L | mutation in subunit DmsA. About 50% of wild-type catalytic efficiency | Escherichia coli |
Q179I | mutation in subunit DmsA. About 500% of wild-type catalytic efficiency | Escherichia coli |
Q179I | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
R149C | mutation in subunit DmsA. About 50% of wild-type catalytic efficiency | Escherichia coli |
R217Q | mutation in subunit DmsA. About 2.7% of wild-type catalytic efficiency | Escherichia coli |
R217Q | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
T148S | mutation in subunit DmsA. About 150% of wild-type catalytic efficiency | Escherichia coli |
T148S | mutation in subunit DmsA. Mutant shows altered kinetic parameters for pyridine N-oxide and dimethylsulfoxide, with Km and kcat decreasing and increasing approximately fourfold,respectively | Escherichia coli |
W191G | mutation in subunit DmsA. About 80% of wild-type catalytic efficiency | Escherichia coli |
W357C | mutation in subunit DmsA. About 100% of wild-type catalytic efficiency | Escherichia coli |
W357F | mutation in subunit DmsA. About 40% of wild-type catalytic efficiency | Escherichia coli |
W357Y | mutation in subunit DmsA. About 60% of wild-type catalytic efficiency | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
reduced benzyl viologen | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.001 | - |
Pyridine N-oxide | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.019 | - |
reduced benzyl viologen | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.019 | - |
reduced benzyl viologen | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.02 | - |
reduced benzyl viologen | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.023 | - |
reduced benzyl viologen | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.024 | - |
Pyridine N-oxide | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.024 | - |
reduced benzyl viologen | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.028 | - |
reduced benzyl viologen | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.028 | - |
Pyridine N-oxide | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.032 | - |
reduced benzyl viologen | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.033 | - |
reduced benzyl viologen | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.034 | - |
Pyridine N-oxide | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.038 | - |
reduced benzyl viologen | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.038 | - |
reduced benzyl viologen | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.039 | - |
reduced benzyl viologen | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.04 | - |
reduced benzyl viologen | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.048 | - |
Pyridine N-oxide | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.048 | - |
Pyridine N-oxide | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.076 | - |
Pyridine N-oxide | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.076 | - |
reduced benzyl viologen | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.095 | - |
Pyridine N-oxide | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.1 | - |
Pyridine N-oxide | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.11 | - |
Pyridine N-oxide | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.11 | - |
reduced benzyl viologen | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.12 | - |
Pyridine N-oxide | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.12 | - |
Pyridine N-oxide | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.14 | - |
Pyridine N-oxide | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.18 | - |
Pyridine N-oxide | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
0.24 | - |
Pyridine N-oxide | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1.1 | - |
reduced benzyl viologen | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
3.8 | - |
Pyridine N-oxide | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylsulfide + H2O + pyridine N-oxide | - |
Escherichia coli | dimethylsulfoxide + pyridine | - |
? | |
dimethylsulfoxide + 2,3-dimethyl-1,4-naphthoquinol | - |
Escherichia coli | dimethylsulfide + H2O + 2,3-dimethyl-1,4-naphthoquinone | - |
r | |
dimethylsulfoxide + reduced benzyl viologen | - |
Escherichia coli | dimethylsulfide + H2O + oxidized benzyl viologen | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | Pyridine N-oxide | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
10 | - |
Pyridine N-oxide | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
14 | - |
reduced benzyl viologen | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
17 | - |
reduced benzyl viologen | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
19 | - |
reduced benzyl viologen | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
23 | - |
reduced benzyl viologen | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
24 | - |
reduced benzyl viologen | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
27 | - |
reduced benzyl viologen | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
33 | - |
Pyridine N-oxide | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
39 | - |
reduced benzyl viologen | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
41 | - |
Pyridine N-oxide | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
46 | - |
reduced benzyl viologen | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
47 | - |
reduced benzyl viologen | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
55 | - |
reduced benzyl viologen | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
60 | - |
reduced benzyl viologen | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
61 | - |
reduced benzyl viologen | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
68 | - |
reduced benzyl viologen | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
95 | - |
Pyridine N-oxide | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
96 | - |
Pyridine N-oxide | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
99 | - |
Pyridine N-oxide | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
99 | - |
Pyridine N-oxide | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
100 | - |
Pyridine N-oxide | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
110 | - |
reduced benzyl viologen | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
110 | - |
Pyridine N-oxide | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
120 | - |
Pyridine N-oxide | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
190 | - |
Pyridine N-oxide | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
190 | - |
Pyridine N-oxide | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
200 | - |
Pyridine N-oxide | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
210 | - |
reduced benzyl viologen | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
260 | - |
Pyridine N-oxide | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
370 | - |
reduced benzyl viologen | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
940 | - |
Pyridine N-oxide | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
Pyridine N-oxide | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
50 | - |
reduced benzyl viologen | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
370 | - |
reduced benzyl viologen | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
760 | - |
reduced benzyl viologen | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
810 | - |
Pyridine N-oxide | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
850 | - |
Pyridine N-oxide | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
900 | - |
reduced benzyl viologen | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
960 | - |
reduced benzyl viologen | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1000 | - |
Pyridine N-oxide | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1000 | - |
reduced benzyl viologen | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1100 | - |
Pyridine N-oxide | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1100 | - |
Pyridine N-oxide | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1100 | - |
Pyridine N-oxide | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1200 | - |
Pyridine N-oxide | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1200 | - |
Pyridine N-oxide | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1300 | - |
Pyridine N-oxide | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1300 | - |
reduced benzyl viologen | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1360 | - |
reduced benzyl viologen | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1600 | - |
reduced benzyl viologen | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1600 | - |
reduced benzyl viologen | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1900 | - |
reduced benzyl viologen | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
1900 | - |
reduced benzyl viologen | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2000 | - |
Pyridine N-oxide | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2100 | - |
Pyridine N-oxide | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
2100 | - |
Pyridine N-oxide | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
3300 | - |
reduced benzyl viologen | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
3400 | - |
reduced benzyl viologen | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4000 | - |
Pyridine N-oxide | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4000 | - |
Pyridine N-oxide | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
5500 | - |
reduced benzyl viologen | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
10000 | - |
Pyridine N-oxide | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
23300 | - |
reduced benzyl viologen | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |