Any feedback?
Literature summary for 1.8.5.4 extracted from
- Purification and characterization of sulfide:quinone oxidoreductase from an acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans (2007), Biosci. Biotechnol. Biochem., 71, 2735-2742.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) or Rosetta-gami(DE3) cells as a thioredoxin-fusion protein in inclusion bodies in an inactive form | Acidithiobacillus ferrooxidans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-n-heptyl-4-hydroxy-quinone-N-oxide | - |
Acidithiobacillus ferrooxidans |  |
| antimycin A | - |
Acidithiobacillus ferrooxidans | |
| cyanide | - |
Acidithiobacillus ferrooxidans | |
| Myxothiazol | - |
Acidithiobacillus ferrooxidans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
ubiquinone-2 | apparent value, at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
| 0.042 | - |
Sulfide | apparent value, at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Acidithiobacillus ferrooxidans | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, SDS-PAGE | Acidithiobacillus ferrooxidans |
| 47290 | - |
x * 47290, deduced from amino acid sequence | Acidithiobacillus ferrooxidans |
| 60000 | 65000 | native enzyme, gel filtration | Acidithiobacillus ferrooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus ferrooxidans | - |
- |
- |
| Acidithiobacillus ferrooxidans NASF-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and TSKgel G3000 gel filtration | Acidithiobacillus ferrooxidans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:sulfur-grown cell | - |
Acidithiobacillus ferrooxidans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
cell-free extract, at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans |
| 20.55 | - |
after 186.8fold purification, at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfide + ubiquinone-2 | - |
Acidithiobacillus ferrooxidans | sulfur + ubiquinol-2 | - |
? | |
| sulfide + ubiquinone-2 | - |
Acidithiobacillus ferrooxidans NASF-1 | sulfur + ubiquinol-2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Acidithiobacillus ferrooxidans |
| ? | x * 47290, deduced from amino acid sequence | Acidithiobacillus ferrooxidans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SQR | - |
Acidithiobacillus ferrooxidans |
| sulfide:quinone oxidoreductase | - |
Acidithiobacillus ferrooxidans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Acidithiobacillus ferrooxidans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Acidithiobacillus ferrooxidans | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Acidithiobacillus ferrooxidans | deduced from amino acid sequence | - |
6.5 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.039 | - |
at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans | Myxothiazol | |
| 0.05 | - |
at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans | 2-n-heptyl-4-hydroxy-quinone-N-oxide | |
| 0.22 | - |
at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans | antimycin A | |
| 0.54 | - |
at pH 7.0, temperature not specified in the publication | Acidithiobacillus ferrooxidans | cyanide | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | SQR is involved in elemental sulfur oxidation in sulfur-grown cells | Acidithiobacillus ferrooxidans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
