Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingobium chlorophenolicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
2-(glutathione-S-yl)-trichloro-p-hydroquinone | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
0.26 | - |
dithiothreitol | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
0.93 | - |
2-mercaptoethanol | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
1.3 | - |
glutathione | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
1.97 | - |
L-cysteine | pH 6.5, 30°C | Sphingobium chlorophenolicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36900 | - |
- |
Sphingobium chlorophenolicum |
65000 | - |
gel filtration | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 2-(glutathione-S-yl)-hydroquinone | Sphingobium chlorophenolicum | - |
glutathione disulfide + hydroquinone | - |
? | |
additional information | Sphingobium chlorophenolicum | PcpF catalyzes glutathione-dependent reduction of glutathionyl-trichloro-p-hydroquinone via a Ping Pong mechanism. First, PcpF reacts with glutathionyl-trichloro-p-hydroquinone to release trichloro-p-hydroquinone and forms a disulfide bond between its Cys53 residue and the glutathione moiety. Then, a glutathione comes in to regenerate PcpF and release glutathione disulfide | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-mercaptoethanol + 2-(glutathione-S-yl)-trichloro-p-hydroquinone | - |
Sphingobium chlorophenolicum | glutathionyl 2-mercaptoethanyl disulfide + trichloro-p-hydroquinone | - |
? | |
dithiothreitol + 2-(glutathione-S-yl)-trichloro-p-hydroquinone | - |
Sphingobium chlorophenolicum | glutathionyl dithiothreityl disulfide + trichloro-p-hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-hydroquinone | - |
Sphingobium chlorophenolicum | glutathione disulfide + hydroquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-trichloro-p-hydroquinone | - |
Sphingobium chlorophenolicum | glutathione disulfide + trichloro-p-hydroquinone | - |
? | |
glutathione + 3-(glutathione-S-yl)-2,6-dichloro-p-hydroquinone | - |
Sphingobium chlorophenolicum | glutathione disulfide + 2,6-dichloro-p-hydroquinone | - |
? | |
L-cysteine + 2-(glutathione-S-yl)-trichloro-p-hydroquinone | - |
Sphingobium chlorophenolicum | glutathionyl cysteinyl disulfide + trichloro-p-hydroquinone | - |
? | |
additional information | PcpF catalyzes glutathione-dependent reduction of glutathionyl-trichloro-p-hydroquinone via a Ping Pong mechanism. First, PcpF reacts with glutathionyl-trichloro-p-hydroquinone to release trichloro-p-hydroquinone and forms a disulfide bond between its Cys53 residue and the glutathione moiety. Then, a glutathione comes in to regenerate PcpF and release glutathione disulfide | Sphingobium chlorophenolicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36900, calculated | Sphingobium chlorophenolicum |
Synonyms | Comment | Organism |
---|---|---|
pcpF | - |
Sphingobium chlorophenolicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
L-cysteine | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
0.89 | - |
dithiothreitol | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
1.09 | - |
2-mercaptoethanol | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
2.08 | - |
glutathione | pH 6.5, 30°C | Sphingobium chlorophenolicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
L-cysteine | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
1.17 | - |
2-mercaptoethanol | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
1.6 | - |
glutathione | pH 6.5, 30°C | Sphingobium chlorophenolicum | |
3.43 | - |
dithiothreitol | pH 6.5, 30°C | Sphingobium chlorophenolicum |