Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.5.7 extracted from

  • Lallement, P.; Meux, E.; Gualberto, J.; Dumarcay, S.; Favier, F.; Didierjean, C.; Saul, F.; Haouz, A.; Morel-Rouhier, M.; Gelhaye, E.; Rouhier, N.; Hecker, A.
    Glutathionyl-hydroquinone reductases from poplar are plastidial proteins that deglutathionylate both reduced and oxidized glutathionylated quinones (2015), FEBS Lett., 589, 37-44.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
structure in complex with glutathione, to 2.5 A resolution. Isoform GHR1 adopts a typical glutathionyl-hydroquinone reductase fold, with a dimerization interface comparable to that of the bacterial and fungal counterparts Populus trichocarpa

Protein Variants

Protein Variants Comment Organism
C49A mutation of catalytic cysteine, loss of activity Populus trichocarpa
C49S mutation of catalytic cysteine, loss of activity Populus trichocarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
2-(glutathione-S-yl)-quercetin wild-type, pH 8.0, 20°C Populus trichocarpa
0.003
-
2-(glutathione-S-yl)-menadiol wild-type, pH 8.0, 20°C Populus trichocarpa
0.072
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone wild-type, pH 8.0, 20°C Populus trichocarpa
0.0926
-
2-(glutathione-S-yl)-1,4-benzoquinone wild-type, pH 8.0, 20°C Populus trichocarpa
0.27
-
2-(glutathione-S-yl)-menadione wild-type, pH 8.0, 20°C Populus trichocarpa

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
Populus trichocarpa 9536
-

Organism

Organism UniProt Comment Textmining
Populus trichocarpa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information primarily expressed in reproductive organs Populus trichocarpa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + (glutathione-S-yl)-2-chloro-1,4-benzoquinone
-
Populus trichocarpa glutathione disulfide + 2-chloro-1,4-benzoquinone
-
?
glutathione + 2'-(glutathione-S-yl)-quercetin
-
Populus trichocarpa glutathione disulfide + quercetin
-
?
glutathione + 2-(glutathione-S-yl)-1,4-benzoquinone
-
Populus trichocarpa glutathione disulfide + 1,4-benzoquinone
-
?
glutathione + 2-(glutathione-S-yl)-menadiol
-
Populus trichocarpa glutathione disulfide + menadiol
-
?
glutathione + 2-(glutathione-S-yl)-menadione
-
Populus trichocarpa glutathione disulfide + menadione
-
?
glutathione + 2-(glutathione-S-yl)-quercetin
-
Populus trichocarpa glutathione disulfide + quercetin
-
r
additional information isoform GHR1 catalyzes the deglutathionylation of both reduced and oxidized glutathionylated quinones, but the enzyme is more catalytically efficient with the reduced forms Populus trichocarpa ?
-
?

Synonyms

Synonyms Comment Organism
GHR1
-
Populus trichocarpa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
2-(glutathione-S-yl)-quercetin wild-type, pH 8.0, 20°C Populus trichocarpa
1.62
-
2-(glutathione-S-yl)-1,4-benzoquinone wild-type, pH 8.0, 20°C Populus trichocarpa
4.75
-
2-(glutathione-S-yl)-menadione wild-type, pH 8.0, 20°C Populus trichocarpa
7.96
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone wild-type, pH 8.0, 20°C Populus trichocarpa
9.42
-
2-(glutathione-S-yl)-menadiol wild-type, pH 8.0, 20°C Populus trichocarpa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.95
-
S-glutathionylquercetin wild-type, pH 8.0, 20°C Populus trichocarpa
17.4
-
2-(glutathione-S-yl)-menadione wild-type, pH 8.0, 20°C Populus trichocarpa
17.5
-
2-(glutathione-S-yl)-1,4-benzoquinone wild-type, pH 8.0, 20°C Populus trichocarpa
110.6
-
(glutathione-S-yl)-2-chloro-1,4-benzoquinone wild-type, pH 8.0, 20°C Populus trichocarpa
3215
-
2-(glutathione-S-yl)-menadiol wild-type, pH 8.0, 20°C Populus trichocarpa