Literature summary for 1.8.5.8 extracted from

Libiad, M.; Yadav, P.; Vitvitsky, V.; Martinov, M.; Banerjee, R.
Organization of the human mitochondrial hydrogen sulfide oxidation pathway (2014), J. Biol. Chem., 289, 30901-30910 .

Search for more literature for 1.8.5.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	Sulfide	with cysteine as acceptor, at pH 7.4 and 37°C	Homo sapiens
0.007	-	Sulfide	with homocysteine as acceptor, at pH 7.4 and 37°C	Homo sapiens
0.01	-	Sulfide	with glutathione as acceptor, at pH 7.4 and 37°C	Homo sapiens
0.13	-	Sulfide	with sulfite as acceptor, at pH 7.4 and 37°C	Homo sapiens
0.22	-	sulfite	at pH 7.4 and 37°C	Homo sapiens
0.32	-	Sulfide	at pH 7.4 and 37°C	Homo sapiens
2	3	cysteine	at pH 7.4 and 37°C	Homo sapiens
22	-	glutathione	at pH 7.4 and 37°C	Homo sapiens
22	-	homocysteine	at pH 7.4 and 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9Y6N5	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography and Mono Q column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydrogen sulfide + cysteine + coenzyme Q1	-	Homo sapiens	?	-	?
hydrogen sulfide + glutathione + coenzyme Q1	-	Homo sapiens	S-sulfanylglutathione + reduced coenzyme Q1	-	?
hydrogen sulfide + homocysteine + coenzyme Q1	-	Homo sapiens	?	-	?
hydrogen sulfide + sulfide + coenzyme Q1	-	Homo sapiens	?	-	?
hydrogen sulfide + sulfite + coenzyme Q1	-	Homo sapiens	thiosulfate + reduced coenzyme Q1	-	?
additional information	no activity with DHLA, cysteamine, coenzyme A, hypotaurine, cysteine sulfinic acid, and thioredoxin	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 48000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
SQR	-	Homo sapiens
sulfide quinone oxidoreductase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
74	-	Sulfide	with homocysteine as acceptor, at pH 7.4 and 37°C	Homo sapiens
92	-	Sulfide	with cysteine as acceptor, at pH 7.4 and 37°C	Homo sapiens
94	-	Sulfide	with glutathione as acceptor, at pH 7.4 and 37°C	Homo sapiens
113	-	Sulfide	with sulfite as acceptor, at pH 7.4 and 37°C	Homo sapiens
373	-	Sulfide	at pH 7.4 and 37°C	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4.1	-	cysteine	at pH 7.4 and 37°C	Homo sapiens
4.2	-	homocysteine	at pH 7.4 and 37°C	Homo sapiens
5.1	-	glutathione	at pH 7.4 and 37°C	Homo sapiens
23.1	-	Sulfide	at pH 7.4 and 37°C	Homo sapiens
1700	-	sulfite	at pH 7.4 and 37°C	Homo sapiens
11000	-	Sulfide	with glutathione as acceptor, at pH 7.4 and 37°C	Homo sapiens
13000	-	Sulfide	with homocysteine as acceptor, at pH 7.4 and 37°C	Homo sapiens
19000	-	Sulfide	with cysteine as acceptor, at pH 7.4 and 37°C	Homo sapiens
29000	-	Sulfide	with sulfite as acceptor, at pH 7.4 and 37°C	Homo sapiens

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Release 2023.1 (January 2023)