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Literature summary for 1.8.5.8 extracted from
- Sulfide quinone oxidoreductase from echiuran worm Urechis unicinctus (2011), Mar. Biotechnol., 13, 93-107 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 109.33% activity at 5 mM | Urechis unicinctus |  |
| glutathione | 110.52% activity at 5 mM | Urechis unicinctus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Urechis unicinctus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 80.32% residual activity at 5 mM | Urechis unicinctus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0156 | - |
ubiquinone | at pH 8.5 and 37°C | Urechis unicinctus | |
| 0.043 | - |
Sulfide | with ubiquinone as cosubstrate, at pH 8.5 and 37°C | Urechis unicinctus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Urechis unicinctus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Urechis unicinctus | H2KXA2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Urechis unicinctus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Urechis unicinctus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| anal sac | - |
Urechis unicinctus | - |
| body wall | - |
Urechis unicinctus | - |
| coelomic fluid | - |
Urechis unicinctus | - |
| epithelium | - |
Urechis unicinctus | - |
| hindgut | - |
Urechis unicinctus | - |
| midgut | highest expression | Urechis unicinctus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.12 | - |
at pH 8.0 and 25°C | Urechis unicinctus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfide + coenzyme Q2 | - |
Urechis unicinctus | ? | - |
? | |
| sulfide + ubiquinone | - |
Urechis unicinctus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Urechis unicinctus |
| ? | x * 50500, calculated from amino acid sequence | Urechis unicinctus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SQR | - |
Urechis unicinctus |
| sulfide:quinone oxidoreductase | - |
Urechis unicinctus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Urechis unicinctus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 42 | more than 60% activity between 27 and 42°C | Urechis unicinctus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Urechis unicinctus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | more than 70% activity between pH 8.0 and 9.0 | Urechis unicinctus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Urechis unicinctus | calculated from amino acid sequence | - |
9 |
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