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Literature summary for 1.8.7.1 extracted from
- Purification and characterization of ferredoxin-sulfite reductase from turnip (Brassica rapa) leaves and comparison of properties with ferredoxin-sulfite reductase from turnip roots (1997), Biosci. Biotechnol. Biochem., 61, 1486-1490.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | 1 mM, added to the reduced enzyme | Brassica rapa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
oxidized ferredoxin | root enzyme | Brassica rapa | |
| 0.013 | - |
reduced ferredoxin | leaf enzyme | Brassica rapa | |
| 0.2 | - |
sulfite | root enzyme | Brassica rapa | |
| 0.22 | - |
sulfite | leaf enzyme | Brassica rapa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
1 * 64000, SDS-PAGE | Brassica rapa |
| 66000 | - |
in presence of 200 mM NaCl, gel filtration | Brassica rapa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + reduced ferredoxin | Brassica rapa | - |
hydrogen sulfide + oxidized ferredoxin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica rapa | - |
cv. Hakutaka | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica rapa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Brassica rapa | - |
| root | - |
Brassica rapa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156 | - |
- |
Brassica rapa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydrogen sulfide + oxidized ferredoxin + H2O | - |
Brassica rapa | sulfite + reduced ferredoxin + H+ | - |
r | |
| sulfite + reduced ferredoxin | - |
Brassica rapa | hydrogen sulfide + oxidized ferredoxin + H2O | - |
? | |
| sulfite + reduced ferredoxin + H+ | - |
Brassica rapa | hydrogen sulfide + oxidized ferredoxin + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000, SDS-PAGE | Brassica rapa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
91% remaining activity of leaf enzyme after heating for 5 min, root enzyme lower heat stability | Brassica rapa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
sulfite | root enzyme | Brassica rapa | |
| 3.6 | - |
reduced ferredoxin | leaf enzyme | Brassica rapa | |
| 6 | - |
reduced ferredoxin | root enzyme | Brassica rapa | |
| 9.1 | - |
sulfite | leaf enzyme | Brassica rapa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
potassium phosphate buffer | Brassica rapa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| siroheme | - |
Brassica rapa | |
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