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Literature summary for 1.8.7.3 extracted from

  • Yan, Z.; Wang, M.; Ferry, J.G.
    A ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase with homologs in the domains Bacteria and Archaea (2017), mBio, 8, e02285-16 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanosarcina acetivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
CoM-S-S-CoB subunit HdrB2C2, at 21┬░C, pH not specified in the publication Methanosarcina acetivorans
1.1
-
CoB subunit HdrB2, at 21┬░C, pH not specified in the publication Methanosarcina acetivorans
1.2
-
CoM subunit HdrB2, at 21┬░C, pH not specified in the publication Methanosarcina acetivorans
1.2
-
CoB subunit HdrB2C2, at 21┬░C, pH not specified in the publication Methanosarcina acetivorans
1.4
-
CoM subunit HdrB2C2, at 21┬░C, pH not specified in the publication Methanosarcina acetivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM Methanosarcina acetivorans
-
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
r
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ Methanosarcina acetivorans
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
Methanosarcina acetivorans reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
r
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
Methanosarcina acetivorans oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
r

Subunits

Subunits Comment Organism
? x * 18000, subunit HdrC2, SDS-PAGE Methanosarcina acetivorans
? x * 33000, subunit HdrB2, SDS-PAGE Methanosarcina acetivorans

Synonyms

Synonyms Comment Organism
HdrB2 subunit Methanosarcina acetivorans
HdrB2C2 subunit Methanosarcina acetivorans

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
Methanosarcina acetivorans