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Literature summary for 1.8.99.2 extracted from

  • Kim, S.; Rahman, A.; Mason, J.T.; Hirasawa, M.; Conover, R.C.; Johnson, M.K.; Miginiac-Maslow, M.; Keryer, E.; Knaff, D.B.; Leustek, T.
    The interaction of 5-adenylylsulfate reductase from Pseudomonas aeruginosa with its substrates (2005), Biochim. Biophys. Acta, 1710, 103-112.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C139S no detectable activity Pseudomonas aeruginosa
C140S 3% activity of the wild type enzyme Pseudomonas aeruginosa
C228S no detectable activity Pseudomonas aeruginosa
C231S no detectable activity Pseudomonas aeruginosa
C256S no detectable activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
thioredoxin thioredoxin originating from Clamydomonas reinhardtii Pseudomonas aeruginosa
8.6
-
thioredoxin thioredoxin originating from Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30470
-
MALDI-TOF mass spectrometry, the enzyme used is a recombinant protein with 48 additional residues containing the His6 tag Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.302
-
mutant enzyme C140S, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS Pseudomonas aeruginosa
10.3
-
wild type enzyme, at 30°C in 100 mM Tris-HCl buffer pH 8.0 containing 1 mM EDTA, 5 mM DTT, 0.106 mM thioredoxin from Escherichia coli, and 0.05 mM APS Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenylylsulfate + thioredoxin
-
Pseudomonas aeruginosa AMP + sulfite + ?
-
?

Subunits

Subunits Comment Organism
monomer SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
5'-adenylylsulfate reductase
-
Pseudomonas aeruginosa
APR
-
Pseudomonas aeruginosa