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Literature summary for 1.8.99.B2 extracted from
- Characterization of a novel thiosulfate dehydrogenase from a marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH (2016), Biosci. Biotechnol. Biochem., 80, 273-278.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-heptyl-4-hydroxy-quinoline N-oxide | 1 microM, presence of 200 mM NaCl, 12% residual activity | Acidithiobacillus thiooxidans |  |
| N-ethylmaleimide | 1 mM, presence of 200 mM NaCl, 84% residual activity | Acidithiobacillus thiooxidans | |
| Sodium sulfate | 100 mM, presence of 200 mM NaCl, 57% residual activity | Acidithiobacillus thiooxidans | |
| sodium sulfite | 0.1 mM, presence of 200 mM NaCl, 45% residual activity | Acidithiobacillus thiooxidans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Acidithiobacillus thiooxidans | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 200 mM, presence of 200 mM NaCl, 86% of the activity in presence of 200 mM NaCl | Acidithiobacillus thiooxidans | |
| MgCl2 | 200 mM, presence of 200 mM NaCl, 68% of the activity in presence of 200 mM NaCl | Acidithiobacillus thiooxidans | |
| NaCl | maximum activity in presence of 200 mM NaCl | Acidithiobacillus thiooxidans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
SDS-PAGE | Acidithiobacillus thiooxidans |
| 99000 | - |
gel filtration | Acidithiobacillus thiooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus thiooxidans | - |
- |
- |
| Acidithiobacillus thiooxidans SH | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 71fold enrichment with a recovery of 4.3% of the total activity | Acidithiobacillus thiooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 thiosulfate + ferricyanide | - |
Acidithiobacillus thiooxidans | tetrathionate + ferrocyanide | - |
? | |
| 2 thiosulfate + ferricyanide | - |
Acidithiobacillus thiooxidans SH | tetrathionate + ferrocyanide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 44000, SDS-PAGE | Acidithiobacillus thiooxidans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thiosulfate dehydrogenase | - |
Acidithiobacillus thiooxidans |
| TSD | - |
Acidithiobacillus thiooxidans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Acidithiobacillus thiooxidans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Acidithiobacillus thiooxidans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | absence of heme groups in the protein | Acidithiobacillus thiooxidans |
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Release 2023.1 (January 2023)
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