BRENDA - Enzyme Database
show all sequences of 1.8.99.B3

Anaerobic sulfatase-maturating enzyme--a mechanistic link with glycyl radical-activating enzymes

Benjdia, A.; Subramanian, S-; Leprince, J.; Vaudry, H.; Johnson, M.K.; Berteau, O.; FEBS J. 277, 1906-1920 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)
Clostridium perfringens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
[4Fe-4S]2+ cluster
three [4Fe-4S]2+ clusters per enzyme molecule
Clostridium perfringens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium perfringens
Q8XMQ3
-
-
Clostridium perfringens type A
Q8XMQ3
-
-
Purification (Commentary)
Commentary
Organism
-
Clostridium perfringens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ac-AVPSCIPSRASILTGM-NH2 + S-adenosyl-L-methionine + dithionite
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
740428
Clostridium perfringens
Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
-
-
-
?
Ac-AVPSCIPSRASILTGM-NH2 + S-adenosyl-L-methionine + dithionite
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
740428
Clostridium perfringens type A
Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
-
-
-
?
additional information
the enzme also perfoms the reaction of the Ser-type anaerobic sulfatase-maturating enzyme, converting a serine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
740428
Clostridium perfringens
?
-
-
-
-
additional information
the enzme also perfoms the reaction of the Ser-type anaerobic sulfatase-maturating enzyme, converting a serine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
740428
Clostridium perfringens type A
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)
Clostridium perfringens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
[4Fe-4S]2+ cluster
three [4Fe-4S]2+ clusters per enzyme molecule
Clostridium perfringens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium perfringens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ac-AVPSCIPSRASILTGM-NH2 + S-adenosyl-L-methionine + dithionite
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
740428
Clostridium perfringens
Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
-
-
-
?
Ac-AVPSCIPSRASILTGM-NH2 + S-adenosyl-L-methionine + dithionite
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
740428
Clostridium perfringens type A
Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
-
-
-
?
additional information
the enzme also perfoms the reaction of the Ser-type anaerobic sulfatase-maturating enzyme, converting a serine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
740428
Clostridium perfringens
?
-
-
-
-
additional information
the enzme also perfoms the reaction of the Ser-type anaerobic sulfatase-maturating enzyme, converting a serine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases
740428
Clostridium perfringens type A
?
-
-
-
-
General Information
General Information
Commentary
Organism
metabolism
involved in the pathway of sulfatase oxidation
Clostridium perfringens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
involved in the pathway of sulfatase oxidation
Clostridium perfringens
Other publictions for EC 1.8.99.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740057
Grove
Further Characterization of Cy ...
Clostridium perfringens, Clostridium perfringens type A
Biochemistry
52
2874-2887
2013
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741331
Goldman
X-ray structure of an AdoMet r ...
Clostridium perfringens
Proc. Natl. Acad. Sci. USA
110
8519-8524
2013
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740428
Benjdia
Anaerobic sulfatase-maturating ...
Clostridium perfringens, Clostridium perfringens type A
FEBS J.
277
1906-1920
2010
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685268
Grove
In vitro characterization of A ...
Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 700721D
Biochemistry
47
7523-7538
2008
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740685
Benjdia
Anaerobic sulfatase-maturating ...
Clostridium perfringens
J. Biol. Chem.
283
17815-26
2008
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1
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740569
Benjdia
Anaerobic sulfatase-maturating ...
Clostridium perfringens
J. Am. Chem. Soc.
129
3462-3463
2007
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