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Literature summary for 1.9.6.1 extracted from

  • Siddiqui, R.A.; Warnecke-Eberz, U.; Hengsberger, A.; Schneider, B.; Kostka, S.; Friedrich, B.
    Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16 (1993), J. Bacteriol., 175, 5867-5876.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
structural genes, napA and napB, are cloned, and their nucleotide sequences is determined Cupriavidus necator

Protein Variants

Protein Variants Comment Organism
additional information the physiological role of NAP in nitrate metabolism is investigated in studies with a mutant bearing a transposon insertion in one of the structural genes Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
nitrate pH 5.5, 37°C Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Cupriavidus necator
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
1 * 17000 + 1 * 87000, SDS-PAGE Cupriavidus necator
18924
-
1 * 93309 + 1 * 18924, calculated from sequence Cupriavidus necator
87000
-
1 * 17000 + 1 * 87000, SDS-PAGE Cupriavidus necator
92000
-
gel filtration Cupriavidus necator
93309
-
1 * 93309 + 1 * 18924, calculated from sequence Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cupriavidus necator an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism ?
-
?
additional information Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism Cupriavidus necator ?
-
?
additional information an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
?
nitrate + reduced benzyl viologen
-
Cupriavidus necator nitrite + oxidized benzyl viologen + H2O
-
?
nitrate + reduced benzyl viologen
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 nitrite + oxidized benzyl viologen + H2O
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 17000 + 1 * 87000, SDS-PAGE Cupriavidus necator
heterodimer 1 * 93309 + 1 * 18924, calculated from sequence Cupriavidus necator

Synonyms

Synonyms Comment Organism
NAP
-
Cupriavidus necator
nitrate reductase, periplasmic
-
Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Cupriavidus necator