Literature summary for 1.97.1.12 extracted from

Mazor, Y.; Borovikova, A.; Caspy, I.; Nelson, N.
Structure of the plant photosystem I supercomplex at 2.6 A resolution (2017), Nat. Plants, 3, 17014 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified plant photosystem I supercomplex including light-harvesting complex I, sitting drop vapour diffusion technique, mixing of 0.006-0.008 ml of PSI protein solution with an equal volume of reservoir solution containing 50 mM di-potassium phosphate, 50 mM Tris, pH 8, 12-17% PEG 400, 1% glycerol, 2 mM L-glutathione, and 0.03% octyl glucose neopentyl glycol, and equilibration against 0.5 ml of reservoir solution, 4°C, 1 month, X-ray diffraction structure determination and analysis at 2.6 A resolution, single-wavelength anomalous diffraction phasing of PSI-LHCI	Pisum sativum

Crystallization (Comment)

Organism

purified plant photosystem I supercomplex including light-harvesting complex I, sitting drop vapour diffusion technique, mixing of 0.006-0.008 ml of PSI protein solution with an equal volume of reservoir solution containing 50 mM di-potassium phosphate, 50 mM Tris, pH 8, 12-17% PEG 400, 1% glycerol, 2 mM L-glutathione, and 0.03% octyl glucose neopentyl glycol, and equilibration against 0.5 ml of reservoir solution, 4°C, 1 month, X-ray diffraction structure determination and analysis at 2.6 A resolution, single-wavelength anomalous diffraction phasing of PSI-LHCI

Pisum sativum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
thylakoid membrane	-	Pisum sativum	42651	-

Organism

Organism	UniProt	Comment	Textmining
Pisum sativum	P17227	var. Kalvadon	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme complex from detergent-treated thylakoid membranes by two steps of anion exchange chromatography with elution through tetraethylammonium chloride followed by precipitation with PEG 6000 and PEG 1500, respectively	Pisum sativum

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Pisum sativum	-

Subunits

Subunits	Comment	Organism
More	the crystal structure reveals the configuration of PsaK, a core subunit important for state transitions in plants, a conserved network of water molecules surrounding the electron transfer centres and an elaborate structure of lipids bridging PSI and its LHCI antenna. The structure of Psak suggests alternative conformations, overview	Pisum sativum

Synonyms

Synonyms	Comment	Organism
PSI	-	Pisum sativum

General Information

General Information	Comment	Organism
additional information	lipid structure and enzyme complex structure analysis of photosystem I supercomplex including light-harvesting complex I, PSI-LHCI, overview. Foerster rate calculations reveal the importance of the luminal-side pigment junctions. Chlorophyll b molecules are coordinated by a mixture of polar and hydrophobic interactions	Pisum sativum
physiological function	photosystem I (PSI) is one of two large reaction centres responsible for converting light photons into the chemical energy needed to sustain life. In the thylakoid membranes of plants, PSI is found together with its integral light-harvesting antenna, light-harvesting complex I (LHCI), in a membrane supercomplex containing hundreds of light-harvesting pigments	Pisum sativum