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Literature summary for 1.97.1.2 extracted from

  • Brune, A.; Schink, B.
    Pyrogallol-to-phloroglucinol conversion and other hydroxyl-transfer reactions catalyzed by cell extracts of Pelobacter acidigallici (1990), J. Bacteriol., 172, 1070-1076.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.97.1.2

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by reducing agents Pelobacter acidigallici

Inhibitors

Inhibitors Comment Organism Structure
additional information oxygen does not inactivate, but the assay requires strict anaerobiosis due to the instability of the substrates and products Pelobacter acidigallici
resorcinol complete inhibition of the reaction of hydroxyhydroquinone with itself Pelobacter acidigallici

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.71
-
 1,2,3,5-Tetrahydroxybenzene
-
 Pelobacter acidigallici

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2,3,5-tetrahydroxybenzene + pyrogallol Pelobacter acidigallici
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pelobacter acidigallici
-
-
-
Pelobacter acidigallici MaGal 2
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen does not inactivate, but the assay requires strict anaerobiosis because of the instability of the substrates and products Pelobacter acidigallici

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
 dimethyl sulfoxide Pelobacter acidigallici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3,5-tetrahydroxybenzene + 1,2,3,5-tetrahydroxybenzene
-
 Pelobacter acidigallici phloroglucinol + pentahydroxybenzene tentatively identified ?
1,2,3,5-tetrahydroxybenzene + 1,2,3,5-tetrahydroxybenzene
-
 Pelobacter acidigallici MaGal 2 phloroglucinol + pentahydroxybenzene tentatively identified ?
1,2,3,5-tetrahydroxybenzene + hydroxyhydroquinone
-
 Pelobacter acidigallici phloroglucinol + 1,2,4,5-tetrahydroxybenzene tentatively identified ?
1,2,3,5-tetrahydroxybenzene + pyrogallol pyrogallol i.e. 1,2,3-tri-hydroxybenzene Pelobacter acidigallici phloroglucinol + 1,2,3,5-tetrahydroxybenzene phloroglucinol i.e. 1,3,5-tri-hydroxybenzene r
1,2,3,5-tetrahydroxybenzene + pyrogallol pyrogallol i.e. 1,2,3-tri-hydroxybenzene Pelobacter acidigallici MaGal 2 phloroglucinol + 1,2,3,5-tetrahydroxybenzene phloroglucinol i.e. 1,3,5-tri-hydroxybenzene r
1,2,3,5-tetrahydroxybenzene + pyrogallol
-
 Pelobacter acidigallici ?
-
 ?
1,2,3,5-tetrahydroxybenzene + resorcinol
-
 Pelobacter acidigallici phloroglucinol + hydroxyhydroquinone
-
 ?
hydroxyhydroquinone + hydroxyhydroquinone
-
 Pelobacter acidigallici resorcinol + 1,2,4,5-tetrahydroxybenzene tentatively identified ?
hydroxyhydroquinone + pyrogallol hydroxyhydroquinone i.e. 1,2,4-trihydroxybenzene Pelobacter acidigallici resorcinol + 1,2,3,5-tetrahydroxybenzene resorcinol i.e. 1,3-benzenediol ?
hydroxyhydroquinone + pyrogallol hydroxyhydroquinone i.e. 1,2,4-trihydroxybenzene Pelobacter acidigallici MaGal 2 resorcinol + 1,2,3,5-tetrahydroxybenzene resorcinol i.e. 1,3-benzenediol ?
additional information dimethyl sulfoxide at unphysiological high concentrations can replace 1,2,3,5-tetrahydroxybenzene with 0.04% efficiency, no transhydroxylation takes place with catechol or phenol + 1,2,3,5-tetrahydroxybenzene, pyrogallol + catechol, resorcinol, hydroquinone or phenol Pelobacter acidigallici ?
-
 ?
additional information 1,2,3,5-tetrahydroxybenzene, 1,2,4-trihydroxybenzene, effective OH-donors Pelobacter acidigallici ?
-
 ?
additional information dimethyl sulfoxide at unphysiological high concentrations can replace 1,2,3,5-tetrahydroxybenzene with 0.04% efficiency, no transhydroxylation takes place with catechol or phenol + 1,2,3,5-tetrahydroxybenzene, pyrogallol + catechol, resorcinol, hydroquinone or phenol Pelobacter acidigallici MaGal 2 ?
-
 ?
additional information 1,2,3,5-tetrahydroxybenzene, 1,2,4-trihydroxybenzene, effective OH-donors Pelobacter acidigallici MaGal 2 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pelobacter acidigallici

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pelobacter acidigallici