BRENDA - Enzyme Database
show all sequences of 1.97.1.4

A dehydroalanyl residue can capture the 5'-deoxyadenosyl radical generated from S-adenosylmethionine by pyruvate formate-lyase-activating enzyme

Wagner, A.F.V.; Demand, J.; Schilling, G.; Pils, T.; Knappe, J.; Biochem. Biophys. Res. Commun. 254, 306-310 (1999)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Reaction
Reaction
Commentary
Organism
S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
mechanism, a deoxyadenosyl radical intermediate, generated by the reductive cleavage of S-adenosylmethionine serves as the actual H atom abstracting species
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
a DELTAAla-containing peptide which lacks hydrogens at the 734-Calpha atom is recognized by the enzyme and is able to trap covalently the nucleophilic 5-deoxyadenosine radical
14935
Escherichia coli
?
-
-
-
-
S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine
-
14935
Escherichia coli
5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl-radical
-
14935
Escherichia coli
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
a DELTAAla-containing peptide which lacks hydrogens at the 734-Calpha atom is recognized by the enzyme and is able to trap covalently the nucleophilic 5-deoxyadenosine radical
14935
Escherichia coli
?
-
-
-
-
S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine
-
14935
Escherichia coli
5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl-radical
-
14935
Escherichia coli
-
Other publictions for EC 1.97.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745175
Shisler
Monovalent cation activation ...
Escherichia coli
J. Am. Chem. Soc.
139
11803-11813
2017
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746477
Al-Bayati
Pneumococcal galactose catabo ...
Streptococcus pneumoniae
Sci. Rep.
7
43587
2017
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744943
Zhang
Functional pyruvate formate l ...
Escherichia coli
FEMS Yeast Res.
15
fov024
2015
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745316
Crain
Pyruvate formate-lyase and it ...
Escherichia coli
J. Biol. Chem.
289
5723-5729
2014
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727087
Crain
Flavodoxin cofactor binding in ...
Escherichia coli
Biochim. Biophys. Acta
1834
2512-2519
2013
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727989
Crain
Pyruvate formate-lyase and its ...
Escherichia coli
J. Biol. Chem.
289
5723-5729
2013
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727665
Dey
S K-edge XAS and DFT calculati ...
Escherichia coli
J. Am. Chem. Soc.
133
18656-18662
2011
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715533
Peng
Pyruvate formate-lyase, eviden ...
Escherichia coli
J. Biol. Chem.
285
27224-27231
2010
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672873
Sparling
Formate synthesis by Clostridi ...
Hungateiclostridium thermocellum, Hungateiclostridium thermocellum 27405
Can. J. Microbiol.
52
681-688
2006
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674782
Atteia
Pyruvate formate-lyase and a n ...
Chlamydomonas reinhardtii
J. Biol. Chem.
281
9909-9918
2006
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662529
Gelius-Dietrich
Pyruvate formate lyase (PFL) a ...
Neocallimastix frontalis
J. Eukaryot. Microbiol.
51
456-463
2004
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13
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663000
Takahashi-Abbe
Biochemical and functional pro ...
Streptococcus mutans
Oral Microbiol. Immunol.
18
293-297
2003
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487237
Asanuma
Molecular characterization and ...
Streptococcus equinus
Appl. Environ. Microbiol.
68
3352-3357
2002
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661924
Krebs
Coordination of adenosylmethio ...
Escherichia coli
J. Am. Chem. Soc.
124
912-913
2002
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660850
Broderick
Pyruvate formate-lyase-activat ...
Escherichia coli
Biochem. Biophys. Res. Commun.
269
451-456
2000
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14935
Wagner
A dehydroalanyl residue can ca ...
Escherichia coli
Biochem. Biophys. Res. Commun.
254
306-310
1999
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14930
Johnson
-
Novel roles for Fe-S clusters ...
Escherichia coli
Pure Appl. Chem.
70
939-946
1998
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14932
Kulzer
Reconstitution and characteriz ...
Escherichia coli
J. Biol. Chem.
273
4897-4903
1998
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6
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6
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14938
Sawers
A glycyl radical solution: oxy ...
Escherichia coli
Mol. Microbiol.
29
945-954
1998
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14939
Hesslinger
Novel keto acid formate-lyase ...
Escherichia coli
Mol. Microbiol.
27
477-492
1998
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14931
Weidner
Molecular characterization of ...
Clostridium pasteurianum
J. Bacteriol.
178
2440-2444
1996
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14933
Frey
Adenosylmethionine-dependent s ...
Escherichia coli
J. Biol. Chem.
269
12432-12437
1994
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14934
Wong
Molecular properties of pyruva ...
Escherichia coli
Biochemistry
32
14102-14110
1993
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14937
Rödel
Primary structures of Escheric ...
Escherichia coli
Eur. J. Biochem.
177
153-158
1988
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14936
Conradt
Pyruvate formate-lyase (inacti ...
Escherichia coli
Arch. Biochem. Biophys.
228
133-142
1984
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