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Literature summary for 2.1.1.113 extracted from

  • Carpenter, M.A.; Bhagwat, A.S.
    DNA base flipping by both members of the PspGI restriction-modification system (2008), Nucleic Acids Res., 36, 5417-5425.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme's catalytic activity is strongly stimulated by addition of Ca2+ or Mg2+, but not by Mn2+, Co2+ or Cd2+ Pyrococcus sp. GI-H

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type enzyme in Escherichia coli Pyrococcus sp. GI-H

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Pyrococcus sp. GI-H
Mg2+ stimulates Pyrococcus sp. GI-H
additional information the enzyme's catalytic activity is strongly stimulated by addition of Ca2+ or Mg2+, but not by Mn2+, Co2+ or Cd2+, however, divalent metal ions are not required for its base-flipping activity Pyrococcus sp. GI-H

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50355, sequence calculation, x * 50000, recombinant enzyme, SDS-PAGE Pyrococcus sp. GI-H
50355
-
x * 50355, sequence calculation, x * 50000, recombinant enzyme, SDS-PAGE Pyrococcus sp. GI-H

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + [DNA]-cytosine Pyrococcus sp. GI-H
-
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus sp. GI-H
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme from Escherichia coli by adsorption chromatography and dialysis to homogeneity Pyrococcus sp. GI-H

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [DNA]-cytosine
-
Pyrococcus sp. GI-H S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?
S-adenosyl-L-methionine + [DNA]-cytosine substrate is methylated plasmid DNA. M.PspGI is a type IIP methyltransferase and enhances fluorescence of 2-aminopurine in DNA if it replaces the second C in the sequence Pyrococcus sp. GI-H S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
?

Subunits

Subunits Comment Organism
? x * 50355, sequence calculation, x * 50000, recombinant enzyme, SDS-PAGE Pyrococcus sp. GI-H

Synonyms

Synonyms Comment Organism
M.PspGI
-
Pyrococcus sp. GI-H
N4-cytosine MTase
-
Pyrococcus sp. GI-H

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Pyrococcus sp. GI-H

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Pyrococcus sp. GI-H

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Pyrococcus sp. GI-H