Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme's catalytic activity is strongly stimulated by addition of Ca2+ or Mg2+, but not by Mn2+, Co2+ or Cd2+ | Pyrococcus sp. GI-H |
Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type enzyme in Escherichia coli | Pyrococcus sp. GI-H |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Pyrococcus sp. GI-H | |
Mg2+ | stimulates | Pyrococcus sp. GI-H | |
additional information | the enzyme's catalytic activity is strongly stimulated by addition of Ca2+ or Mg2+, but not by Mn2+, Co2+ or Cd2+, however, divalent metal ions are not required for its base-flipping activity | Pyrococcus sp. GI-H |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50355, sequence calculation, x * 50000, recombinant enzyme, SDS-PAGE | Pyrococcus sp. GI-H |
50355 | - |
x * 50355, sequence calculation, x * 50000, recombinant enzyme, SDS-PAGE | Pyrococcus sp. GI-H |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [DNA]-cytosine | Pyrococcus sp. GI-H | - |
S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. GI-H | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme from Escherichia coli by adsorption chromatography and dialysis to homogeneity | Pyrococcus sp. GI-H |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [DNA]-cytosine | - |
Pyrococcus sp. GI-H | S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? | |
S-adenosyl-L-methionine + [DNA]-cytosine | substrate is methylated plasmid DNA. M.PspGI is a type IIP methyltransferase and enhances fluorescence of 2-aminopurine in DNA if it replaces the second C in the sequence | Pyrococcus sp. GI-H | S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50355, sequence calculation, x * 50000, recombinant enzyme, SDS-PAGE | Pyrococcus sp. GI-H |
Synonyms | Comment | Organism |
---|---|---|
M.PspGI | - |
Pyrococcus sp. GI-H |
N4-cytosine MTase | - |
Pyrococcus sp. GI-H |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus sp. GI-H |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Pyrococcus sp. GI-H |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Pyrococcus sp. GI-H |