Cloned (Comment) | Organism |
---|---|
gene CaMTL2, cDNA library construction, DNA and amino acid sequence determination and analysis | Coffea arabica |
gene CaMXMT, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species, expression of CaMXMT as GST-fusion enzyme in Escherichia coli strain JM109 | Coffea arabica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Coffea arabica | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | second step in caffeine biosynthesis pathway | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | second step in caffeine biosynthesis pathway from xanthosine | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | Q9AVJ9 | gene CaMXMT, enzyme MXMT1; var. caturra | - |
Coffea arabica | Q9AVK1 | gene CaMTL2; var. caturra | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography | Coffea arabica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | - |
Coffea arabica | - |
leaf | high expression level of MXMT in young leaves, low expression level in old leaves | Coffea arabica | - |
leaf | old and young | Coffea arabica | - |
additional information | wide tissue distribution | Coffea arabica | - |
root | - |
Coffea arabica | - |
root | low expression level of MXMT | Coffea arabica | - |
stem | - |
Coffea arabica | - |
stem | high expression level of MXMT | Coffea arabica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway from xanthosine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
S-adenosyl-L-methionine + paraxanthine | low activity | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir |
Synonyms | Comment | Organism |
---|---|---|
7-methylxanthine methyltransferase | - |
Coffea arabica |
7-methylxanthine N-methyltransferase | - |
Coffea arabica |
MXMT | - |
Coffea arabica |
MXMT1 | - |
Coffea arabica |
N-methyltransferase | - |
Coffea arabica |