Cloned (Comment) | Organism |
---|---|
Agrobacterium tumefaciens-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis suign the RNAi construct, pFGC1008-CS, method, overview | Camellia sinensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Agrobacterium tumefaciens-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis suign the RNAi construct, pFGC1008-CS. The pFGC1008-CS contained 376 bp of caffeine synthase cDNA fragment in sense and antisense direction with an intron in between. Marked reduction in caffeine and theobromine contents in young shoots of tea seedlings occur after root transformation | Camellia sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Camellia sinensis | tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine | ? | - |
? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | Camellia sinensis | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | Camellia sinensis | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | B9VI89 | cv. Kangra jat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
root | - |
Camellia sinensis | - |
seedling | - |
Camellia sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine | Camellia sinensis | ? | - |
? | |
S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Camellia sinensis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Camellia sinensis | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Camellia sinensis |