Application | Comment | Organism |
---|---|---|
food industry | producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA | Camellia sinensis |
Cloned (Comment) | Organism |
---|---|
semiquantitative-PCR for caffeine synthase transcript expression analysis | Camellia sinensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isolation of a partial fragment of caffeine synthase from cv. Kangra jat and usage for design an RNAi construct, pFGC1008-CS, detection of siRNAs specific to caffeine synthase in transgenic plants. Transformation of somatic embryos with the developed construct using a biolistic method. Transformed somatic embryos show reduction in the levels of caffeine synthase transcript expression as well as in caffeine content. Transgenic plants generated from the transformed somatic embryos show a significant suppression of caffeine synthase transcript expression and also a reduction of 44-61% in caffeine and 46-67% in theobromine contents as compared to the controls | Camellia sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,7-dimethylxanthine | Camellia sinensis | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | Camellia sinensis | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | - |
cv. Kangra jat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
seedling | - |
Camellia sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Camellia sinensis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
S-adenosyl-L-methionine + 7-methylxanthine | - |
Camellia sinensis | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3, 7-dimethylxanthine methyltransferase | - |
Camellia sinensis |
7-N-methylxanthine methyltransferase | - |
Camellia sinensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Camellia sinensis |
General Information | Comment | Organism |
---|---|---|
malfunction | post-transcriptional silencing of caffeine synthase mRNA leads to reduced caffeine content in tea leaves | Camellia sinensis |
metabolism | caffeine synthase is a bifunctional enzyme that catalyzes the last two steps of caffeine biosynthesis in tea as 7-N-methylxanthine methyltransferase and 3,7-dimethylxanthine methyltransferase | Camellia sinensis |