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Literature summary for 2.1.1.161 extracted from

  • Waditee, R.; Tanaka, Y.; Aoki, K.; Hibino, T.; Jikuya, H.; Takano, J.; Takabe, T.
    Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica (2003), J. Biol. Chem., 278, 4932-4942.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Aphanothece halophytica

Protein Variants

Protein Variants Comment Organism
M172R almost inactive Aphanothece halophytica
P171Q strongly increased Km for dimethylglycine, slight increase in the Km for S-adenosyl-L-methionine Aphanothece halophytica
P171Q/M172R almost inactive Aphanothece halophytica

Inhibitors

Inhibitors Comment Organism Structure
alanine slight inhibition Aphanothece halophytica
cysteine slight inhibition Aphanothece halophytica
isovaleric acid slight inhibition Aphanothece halophytica
KCl
-
Aphanothece halophytica
methionine slight inhibition Aphanothece halophytica
additional information no inhibition with betaine Aphanothece halophytica
n-butyric acid
-
Aphanothece halophytica
N-methylglycine slight inhibition Aphanothece halophytica
NaCl
-
Aphanothece halophytica
phenylalanine slight inhibition Aphanothece halophytica
propionic acid slight inhibition Aphanothece halophytica
S-adenosyl-L-homocysteine
-
Aphanothece halophytica
t-butyrylacetic acid
-
Aphanothece halophytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
S-adenosyl-L-methionine pH 8.8, 37°C Aphanothece halophytica
0.5
-
N,N-dimethylglycine pH 8.8, 37°C Aphanothece halophytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
gel filtration Aphanothece halophytica
31000
-
1 * 31000, SDS-PAGE, 1 * 29000, gel filtration Aphanothece halophytica
31210
-
calculated from the deduced amino acid sequence Aphanothece halophytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + N,N-dimethylglycine Aphanothece halophytica synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
?

Organism

Organism UniProt Comment Textmining
Aphanothece halophytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aphanothece halophytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N,N-dimethylglycine
-
Aphanothece halophytica S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
?
S-adenosyl-L-methionine + N,N-dimethylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Aphanothece halophytica S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE, 1 * 29000, gel filtration Aphanothece halophytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Aphanothece halophytica

pH Range

pH Minimum pH Maximum Comment Organism
7 11 very low activity below pH 7 Aphanothece halophytica