Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Aphanothece halophytica |
Protein Variants | Comment | Organism |
---|---|---|
M172R | almost inactive | Aphanothece halophytica |
P171Q | strongly increased Km for dimethylglycine, slight increase in the Km for S-adenosyl-L-methionine | Aphanothece halophytica |
P171Q/M172R | almost inactive | Aphanothece halophytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alanine | slight inhibition | Aphanothece halophytica | |
cysteine | slight inhibition | Aphanothece halophytica | |
isovaleric acid | slight inhibition | Aphanothece halophytica | |
KCl | - |
Aphanothece halophytica | |
methionine | slight inhibition | Aphanothece halophytica | |
additional information | no inhibition with betaine | Aphanothece halophytica | |
n-butyric acid | - |
Aphanothece halophytica | |
N-methylglycine | slight inhibition | Aphanothece halophytica | |
NaCl | - |
Aphanothece halophytica | |
phenylalanine | slight inhibition | Aphanothece halophytica | |
propionic acid | slight inhibition | Aphanothece halophytica | |
S-adenosyl-L-homocysteine | - |
Aphanothece halophytica | |
t-butyrylacetic acid | - |
Aphanothece halophytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C | Aphanothece halophytica | |
0.5 | - |
N,N-dimethylglycine | pH 8.8, 37°C | Aphanothece halophytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Aphanothece halophytica |
31000 | - |
1 * 31000, SDS-PAGE, 1 * 29000, gel filtration | Aphanothece halophytica |
31210 | - |
calculated from the deduced amino acid sequence | Aphanothece halophytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + N,N-dimethylglycine | Aphanothece halophytica | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aphanothece halophytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aphanothece halophytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + N,N-dimethylglycine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? | |
S-adenosyl-L-methionine + N,N-dimethylglycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE, 1 * 29000, gel filtration | Aphanothece halophytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Aphanothece halophytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | very low activity below pH 7 | Aphanothece halophytica |