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Literature summary for 2.1.1.166 extracted from

  • Hager, J.; Staker, B.L.; Bugl, H.; Jakob, U.
    Active site in RrmJ, a heat shock-induced methyltransferase (2002), J. Biol. Chem., 277, 41978-41986.
    View publication on PubMed
Search for more literature for 2.1.1.166

Protein Variants

Protein Variants Comment Organism
D124A the mutant D124A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo Escherichia coli
D83A the mutant D83A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo Escherichia coli
E199A the RrmJ deletion strains expressing the E199A variant protein shows only slight growth defects, indicating that the residue is not as important in the catalytic mechanism Escherichia coli
K164A the mutant D83A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo Escherichia coli
K38A the mutant D83A is unable to rescue the growth defect of the rrmJ deletion strain, indicating that this mutation causes the inactivation of RrmJ in vivo Escherichia coli
Y201A the RrmJ deletion strains expressing the Y201A variant protein shows only slight growth defects, indicating that the residue is not as important in the catalytic mechanism Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information apparent Km values for 50S ribosomal subunits. The apparent Km value for 23S rRNA within 50S ribosomal subunits is 0.0008 mM at 37°C Escherichia coli
0.0037
-
 S-adenosyl-L-methionine pH 7.5, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine2552 in 23S rRNA Escherichia coli the 2'-O-ribose methylation of the universally conserved base U2552 in the A-loop of the 23 S rRNA S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140N5X9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + uridine2552 in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA a reaction mechanism for the methyltransfer activity of RrmJ is proposed Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uridine2552 in 23S rRNA the 2'-O-ribose methylation of the universally conserved base U2552 in the A-loop of the 23 S rRNA Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + uridine2552 in 23S rRNA the 2'-O-ribose methylation of the universally conserved base U2552 in the A-loop of the 23 S rRNA. The active site of RrmJ appears to be formed by a catalytic triad consisting of two lysine residues, Lys-38 and Lys-164, and the negatively charged residue Asp-124. Another highly conserved residue, Glu-199, that is present in the active site of RrmJ and VP39 appears to play only a minor role in the methyltransfer reaction in vivo. A reaction mechanism for the methyltransfer activity of RrmJ is proposed Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
FTSJ
-
 Escherichia coli
heat shock protein RrmJ
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60 50°C: about 75% of maximal activity, 60°C: about 95% of maximal activity Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 RrmJ is a thermostable heat shock protein Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the apparent kcat value for 23 S rRNA within 50S ribosomal subunits is 0.001/sec mM at 37°C Escherichia coli
0.001
-
 S-adenosyl-L-methionine pH 7.5, 37°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction absence of this methylation, which occurs late in the maturation process of the ribosome, appears to cause the destabilization and premature dissociation of the 50 S ribosomal subunit Escherichia coli