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Literature summary for 2.1.1.228 extracted from

  • Christian, T.; Evilia, C.; Williams, S.; Hou, Y.M.
    Distinct origins of tRNA(m1G37) methyltransferase (2004), J. Mol. Biol., 339, 707-719.
    View publication on PubMed
Search for more literature for 2.1.1.228

Application

Application Comment Organism
medicine the sequence and structural distinction of bacterial TrmD from the eukaryotic Trm5 suggests that strategies can be developed to specifically inhibit TrmD without a deleterious effect on humans Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
D201A 59% activity realtive to the wild-type Methanocaldococcus jannaschii
D223A 6.2% activity realtive to the wild-type Methanocaldococcus jannaschii
G205A/G207A 12.3% activity realtive to the wild-type Methanocaldococcus jannaschii
N265A 11.2% activity realtive to the wild-type Methanocaldococcus jannaschii
P267A 1.3% activity realtive to the wild-type Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58293
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is specific for methylation of guanine37 in tRNA. No methylation of tRNAArg(UCU) possessing the sequence U36G37 Methanocaldococcus jannaschii ?
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAArg(UCG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAArg(UCG)
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNACys(GCA) possessing the sequence A36G37. The enzyme is inactive with mutant forms of Methanocaldococcus jannaschii tRNACys(GCA) containing A37, C37, or U37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNACys(GCA)
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAGlu(UUC) possessing the sequence C36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAGlu(UUC)
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNALeu(UCG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNALeu(UCG)
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro(GGG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro(GGG)
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro(UGG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro(UGG)
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
MJ0883
-
 Methanocaldococcus jannaschii
tRNA(m1G37) methyltransferase
-
 Methanocaldococcus jannaschii
tRNA(m1G37) MTase
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Methanocaldococcus jannaschii