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Literature summary for 2.1.1.228 extracted from

  • Christian, T.; Hou, Y.M.
    Distinct determinants of tRNA recognition by the TrmD and Trm5 methyl transferases (2007), J. Mol. Biol., 373, 623-632.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.228

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families Escherichia coli
additional information
-
 additional information kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families Methanocaldococcus jannaschii
0.0012
-
 guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 52°C Methanocaldococcus jannaschii
0.0028
-
 guanine37 in Escherichia coli tRNAPro pH 8.0, 37°C Escherichia coli
0.0073
-
 guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine37 in tRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Methanocaldococcus jannaschii Q58293
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNAPro
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNAPro
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro
-
 ?
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro
-
 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Synonyms

Synonyms Comment Organism
TRM5
-
 Methanocaldococcus jannaschii
TrmD
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assas at Escherichia coli
52
-
 assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families Escherichia coli
additional information
-
 additional information kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families Methanocaldococcus jannaschii
0.0086
-
 guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 52°C Methanocaldococcus jannaschii
0.01
-
 guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 37°C Escherichia coli
0.017
-
 guanine37 in Escherichia coli tRNAPro pH 8.0, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli
8
-
 assay at Methanocaldococcus jannaschii

General Information

General Information Comment Organism
physiological function the m1G37 modification prevents tRNA frameshifts on the ribosome by assuring correct codon-anticodon pairings, and thus is essential for the fidelity of protein synthesis Escherichia coli
physiological function the m1G37 modification prevents tRNA frameshifts on the ribosome by assuring correct codon-anticodon pairings, and thus is essential for the fidelity of protein synthesis Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families Escherichia coli
additional information
-
 additional information kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families Methanocaldococcus jannaschii
1.7
-
 guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 37°C Escherichia coli
6.1
-
 guanine37 in Escherichia coli tRNAPro pH 8.0, 37°C Escherichia coli
7.22
-
 guanine37 in Methanocaldococcus jannaschii tRNAPro pH 8.0, 52°C Methanocaldococcus jannaschii