Cloned (Comment) | Organism |
---|---|
gene trmD, recombinant expression of His-tagged isolated N-terminal domain in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the N-terminal domain is a useful construct to probe the molecular interactions with SAM competitive inhibitors, in the search for molecules with antibiotic activity | Pseudomonas aeruginosa | |
sinefungin | an active-site inhibitor and S-adenosyl-L-methionine (SAM) analogue, that binds at the N-terminal dommain in the SAM binding site. Structure analysis of crystallized isolated dimeric NTD, overview | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Pseudomonas aeruginosa | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Pseudomonas aeruginosa UCBPP-PA14 | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q02RL6 | - |
- |
Pseudomonas aeruginosa UCBPP-PA14 | Q02RL6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isolated N-terminal domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Pseudomonas aeruginosa | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Pseudomonas aeruginosa UCBPP-PA14 | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bacterial tRNA-(N1G37) methyltransferase | - |
Pseudomonas aeruginosa |
TrmD | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
metabolites such as S-adenosyl-L-methionine (SAM), S-adenosyl-L-homocysteine (SAH), and methylthioadenosine (MTA) enhance the thermostability of the enzyme's N-terminal domain by increasing its melting temperature (Tm) | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | SAM | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
additional information | the N-terminal domain (NTD) contains the S-adenosyl-L-methionine (SAM) binding region, this domain binds to SAM, S-adenosyl-L-homocysteine (SAH), and active-site inhibitors such as the SAM analogue sinefungin. Metabolites such as SAM, SAH and MTA enhance the thermostability of NTD by increasing its melting temperature (Tm), dynamics and ligand binding of NTD | Pseudomonas aeruginosa |