Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
3 * 52000 | Methanosarcina barkeri |
170000 | - |
- |
Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylamine + a [Co(I) methylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native MtmB from monomethylamine-grown cells, by two different steps of anionexchange chromatography, followed by gel filtration and another step of anion exchange chromatography to near homogeneity | Methanosarcina barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.49 | - |
purified native enzyme, pH 7.0, 37°C | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylamine + a [Co(I) methylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 52000 | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
MMA:MMCP methyltransferase | - |
Methanosarcina barkeri |
MMAMT | - |
Methanosarcina barkeri |
monomethylamine:coenzyme M methyl transferase | - |
Methanosarcina barkeri |
monomethylamine:MMCP methyltransferase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
physiological function | the MMAMT polypeptide is rate-limiting for methyl transfer until at a 2fold molar excess over monomethylamine corrinoid protein, MMCP. MMAMT and MMCP form a complex. MMCP is the major corrinoid protein for methanogenesis from monomethylamine | Methanosarcina barkeri |