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Literature summary for 2.1.1.273 extracted from

  • Chen, F.; D'Auria, J.C.; Tholl, D.; Ross, J.R.; Gershenzon, J.; Noel, J.P.; Pichersky, E.
    An Arabidopsis thaliana gene for methylsalicylate biosynthesis, identified by a biochemical genomics approach, has a role in defense (2003), Plant J., 36, 577-588.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alamethicin treatment of detached Arabidopsis thaliana leaves efficiently induced methyl salicylate but not methyl benzoate emission Arabidopsis thaliana
insect herbivory feeding by the larvae of the diamondback moth on rosette leaves of intact plants resultes in low methyl salicylate and very low methyl benzoate emission Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli Arabidopsis lyrata
coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
benzoic acid apparent Km-value, pH 7.7, 30°C Arabidopsis thaliana
0.067
-
S-adenosyl-L-methionine apparent Km-value, pH 7.7, 30°C Arabidopsis thaliana
0.087
-
S-adenosyl-L-methionine apparent Km-value, pH 7.7, 30°C Arabidopsis lyrata
0.131
-
benzoic acid apparent Km-value, pH 7.7, 30°C Arabidopsis lyrata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43360
-
AlBSMT1 subunit, calculated from sequence Arabidopsis lyrata
43363
-
2 * 44000, SDS–PAGE, 2 * 43363 calculated from sequence Arabidopsis lyrata
43365
-
2 * 44000, SDS–PAGE, 2 * 43365 calculated from sequence Arabidopsis thaliana
43370
-
subunit, calculated from sequence Arabidopsis thaliana
44000
-
AlBSMT1 subunit mass by SDS–PAGE Arabidopsis lyrata
44000
-
subunit mass by SDS–PAGE Arabidopsis thaliana
91000
-
AlBSMT1 holoenzyme, estimated by size exclusion chromatography Arabidopsis lyrata
91000
-
holoenzyme, estimated by size exclusion chromatography Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis lyrata Q6XMI1
-
-
Arabidopsis thaliana Q6XMI3
-
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography Arabidopsis thaliana
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography Arabidopsis lyrata

Source Tissue

Source Tissue Comment Organism Textmining
flower AlBSMT1 transcript levels are, similarly to AtBSMT1 transcripts in Arabidopsis thaliana higher in flowers Arabidopsis lyrata
-
flower highest levels of AtBSMT1 transcript Arabidopsis thaliana
-
leaf detectable AlBSMT1 transcript levels Arabidopsis lyrata
-
leaf lower levels of AtBSMT1 transcript Arabidopsis thaliana
-
additional information little or no AtBSMT1 transcripts observed in roots or siliques of plants Arabidopsis thaliana
-
stem lower levels of AtBSMT1 transcript Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in contrast to AtBSMT1 no activity with 1 mM 3-hydroxybenzoic acid Arabidopsis lyrata ?
-
?
additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.274, salicylate 1-O-methyltransferase Arabidopsis thaliana ?
-
?
additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.274, salicylate 1-O-methyltransferase Arabidopsis lyrata ?
-
?
S-adenosyl-L-methionine + 3-hydroxybenzoic acid 54% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
?
S-adenosyl-L-methionine + anthranilic acid 32% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl anthranilate
-
?
S-adenosyl-L-methionine + anthranilic acid 35% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl anthranilate
-
?
S-adenosyl-L-methionine + benzoic acid
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl benzoate
-
?
S-adenosyl-L-methionine + benzoic acid
-
Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl benzoate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 44000, SDS–PAGE, 2 * 43363 calculated from sequence Arabidopsis lyrata
homodimer 2 * 44000, SDS–PAGE, 2 * 43365 calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AlBSMT1
-
Arabidopsis lyrata
AtBSMT1
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
benzoic acid pH 7.7, 30°C Arabidopsis thaliana
0.3
-
benzoic acid pH 7.7, 30°C Arabidopsis lyrata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Arabidopsis thaliana
7 8
-
Arabidopsis lyrata

Expression

Organism Comment Expression
Arabidopsis thaliana under normal growing conditions AtBSMT1 promoter is only active in leaves at the base of the trichomes, in the hydathodes, and in the upper part of the sepals of flowers. Strong AtBSMT1 promoter activity is observed around lesions in the leaves caused by thrips of the genus Frankliniella additional information
Arabidopsis thaliana leaf wounding and uprooting causes AtBSMT1 transcripts to accumulate quickly within 2 h after treatment with uprooting being more effective up
Arabidopsis thaliana methyl jasmonate treatment, in which plants are exposed to methyl jasmonate vapors in a closed container, results in slow induction up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.9
-
benzoic acid pH 7.7, 30°C Arabidopsis thaliana
9.6
-
benzoic acid pH 7.7, 30°C Arabidopsis lyrata