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Literature summary for 2.1.1.273 extracted from

  • Han, X.; Yang, Q.; Liu, Y.; Yang, Z.; Wang, X.; Zeng, Q.; Yang, H.
    Evolution and function of the Populus SABATH family reveal that a single amino acid change results in a substrate switch (2018), Plant Cell Physiol., 59, 392-403 .
    View publication on PubMed
Search for more literature for 2.1.1.273

Cloned(Commentary)

Cloned (Comment) Organism
gene SABATH24, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overview, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Populus trichocarpa

Protein Variants

Protein Variants Comment Organism
H157M site-directed mutagenesis, this mutation results in a switch from a preference for benzoic acid (BA) over salicylic acid (SA) in wild-type PtSABATH24 to a preference for SA over BA in the H157M mutant Populus trichocarpa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + benzoate Populus trichocarpa
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
S-adenosyl-L-methionine + salicylate Populus trichocarpa
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Populus trichocarpa B9IPD3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Populus trichocarpa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56.97
-
 recombinant wild-type PtSABATH24 with benzoic acid, pH 7.4, 25°C Populus trichocarpa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme PtSABATH24 has a wide substrate spectrum, exhibiting enzymatic activity towards eight of the substrates tested, i.e. indole-3-acetic acid, benzoic acid, salicylic acid, vanillic acid, farnesoic acid, nicotinic acid, coumalic acid, and trans-cinnamic acid, with the highest enzymatic activity towards benzoic acid. Compared with other Populus SABATH proteins, PtSABATH24 shows at least 42.5fold higher enzymatic activity towards benzoic acid Populus trichocarpa ?
-
-
S-adenosyl-L-methionine + benzoate
-
 Populus trichocarpa methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
S-adenosyl-L-methionine + salicylate
-
 Populus trichocarpa methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
class II SABATH protein
-
 Populus trichocarpa
More see also EC 2.1.1.274 Populus trichocarpa
PtSABATH24
-
 Populus trichocarpa
S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase UniProt Populus trichocarpa
SABATH methyltransferase 4 UniProt Populus trichocarpa
SABATH24
-
 Populus trichocarpa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Populus trichocarpa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Populus trichocarpa

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Populus trichocarpa

General Information

General Information Comment Organism
evolution the enzyme belongs to the SABATH family, phylogenetic analysis and tree, detailed overview. Twenty-eight Populus SABATH genes are divided into three classes with distinct divergences in their gene structure, expression responses to abiotic stressors and enzymatic properties of encoded proteins. Populus class I SABATH proteins convert indole-3-acetic acid (IAA) to methyl-IAA, class II SABATH proteins convert benzoic acid (BA) and salicylic acid (SA) to methyl-BA and methyl-SA, while class III SABATH proteins convert farnesoic acid (FA) to methyl-FA. For Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. Of the Populus SABATH class II proteins, PtSABATH4 and 24 show the highest activity towards SA and BA, respectively Populus trichocarpa
malfunction for Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. Mutation of His157 of PtSABATH24 to a methionine residue also results in a switch from a preference for BA over SA in wild-type PtSABATH24 to a preference for SA over BA in the H157M mutant. The mutation M156H in PtSBATH4 (EC 2.1.1.274) results in a switch from a preference for salicylic acid (SA) over benzoic acid (BA) in wild-type PtSABATH4 to a preference for BA over SA in the M156H mutant Populus trichocarpa
metabolism expression patterns of Populus SABATH genes under normal growth conditions and abiotic stress, overview Populus trichocarpa