Literature summary for 2.1.1.274 extracted from

Wang, G.; Li, Q.; Wang, C.; Jin, C.; Ji, J.; Guan, C.
A salicylic acid carboxyl methyltransferase-like gene LcSAMT from Lycium chinense, negatively regulates the drought response in transgenic tobacco (2019), Environ. Exp. Bot., 167, 103833 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene SAMT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Escherichia coli, and functional recombinant expression in transgenic Nicotiana tabacum plants via Agrobacterium tunefaciens strain EHA105 transfection method, overexpression of LcSAMT gene markedly enhances the methylsalicylate content and reduces the accumulation of salicylate in transgenic tobacco plants	Lycium chinense

Cloned (Comment)

Organism

gene SAMT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Escherichia coli, and functional recombinant expression in transgenic Nicotiana tabacum plants via Agrobacterium tunefaciens strain EHA105 transfection method, overexpression of LcSAMT gene markedly enhances the methylsalicylate content and reduces the accumulation of salicylate in transgenic tobacco plants

Lycium chinense

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + salicylate	Lycium chinense	-	methyl salicylate + S-adenosyl-L-homocysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Lycium chinense	A0A514TTE2	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	high expression level	Lycium chinense	-
root	low expression level	Lycium chinense	-
stem	moderate expression level	Lycium chinense	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + salicylate	-	Lycium chinense	methyl salicylate + S-adenosyl-L-homocysteine	-	?

Subunits

Subunits	Comment	Organism
?	x * 39930, sequence calculation, x * 40000, recombinant His-tagged enzyme, SDS-PAGE	Lycium chinense

Synonyms

Synonyms	Comment	Organism
LcSAMT	-	Lycium chinense
salicylic acid carboxyl methyltransferase-like	-	Lycium chinense
SAMT	-	Lycium chinense

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Lycium chinense

pI Value

Organism	Comment	pI Value Maximum	pI Value
Lycium chinense	sequence calculation	-	5.13

Expression

Organism	Comment	Expression
Lycium chinense	expression of LcSAMT gene is observed to be elevated in Lycium chinense under drought stress treatment, PEG 6000 treatment transiently induces the enzyme peaking after 12 h	up

General Information

General Information	Comment	Organism
malfunction	overexpression of LcSAMT gene markedly enhances the methylsalicylate (MeSA) content and reduces the accumulation of salicylate (SA) in transgenic tobacco plants, the conversion of MeSA from SA leads to the depletion of the free SA pool. Overexpression of LcSAMT gene in tobacco significantly increases sensitivity of transgenic plants to drought stress, probably due to the decreased SA accumulation. Increased accumulation of ROS, elevated MDA levels, reduced proline contents, and lowered expression of APX, CAT and SOD genes are also observed in the LcSAMT transgenic tobacco plants under drought stress, which means that the LcSAMT-overexpressing transgenic tobacco plants have decreased resistance to oxidative stress in comparison with control plants under drought stress. LcSAMT-overexpressing transgenic tobacco plants display decreased abscisic acid (ABA) accumulation and reduced transcript expression of NtNCED1 and NtRD22 genes. Therefore, the increased sensitivity of transgenic plants overexpressing LcSAMT gene to drought stress might also act through an ABA-dependent pathway. Overexpression of LcSAMT decreases RWC, proline, chlorophyll content, and the photosynthetic capacity, and increases MDA content of transgenic Nicotiana tabacum plants exposed to drought stress	Lycium chinense
additional information	analysis of molecular mechanism of LcSAMT gene	Lycium chinense
physiological function	salicylic acid (SA) is a phenolic compound involved in plant growth and development. Salicylic acid carboxyl methyltransferase (SAMT) can catalyze the methylation of SA with S-adenosyl-L-methionine as the methyl donor to form methyl salicylate (MeSA). Recombinant salicylic acid carboxyl methyltransferase-like gene LcSAMT from Lycium chinense negatively regulates the drought response in transgenic tobacco. Enzyme LcSAMT regulates the expression of stress-related genes in transgenic Nicotiana tabacum plants exposed to drought stress	Lycium chinense